Effect of over-expression of bacterial ribonuclease H on the utility of antisense MYC oligodeoxynucleotides in the monocytic leukemia cell line U937

A. Rosolen, E. Kyle, C. Chavany, Raymond Bergan, E. T. Kalman, R. Crouch, L. Neckers

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

RNase H has been clearly implicated in vitro in mediating some antisense effects. In vivo evidence is limited to experiments performed in Xenopus oocytes in which antisense oligonucleotides are microinjected. In other mammalian cell systems scant data have been obtained to support or deny a role for RNase H as antisense mediator in vivo. These experiments were designed to test the hypothesis that RNase H mediates the MYC antisense-induced reduction in MYC protein observed in the human monocytic leukemia cell line U937. A bacterial RNase H-containing episomal replicon was constructed and stable transfectants were obtained which expressed E coli RNase H in their cytoplasm at a 10-fold higher level than endogenous RNase H. These cells failed to demonstrate hightened sensitivity to MYC antisense (phosphorothioate, end capped and phosphodiester) compared with untransfected or E coli RNase H antisense tranfected cells. PCR analysis of each transfectant treated and untreated with MYC antisense failed to demonstrate the appearance of truncated MYC mRNA. These results do not support a role for RNase H in the mediation of MYC antisense-induced MYC protein reduction and growth inhibition in U937 cells.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalBiochimie
Volume75
Issue number1-2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Ribonuclease H
Oligodeoxyribonucleotides
Leukemia
Cells
Cell Line
Escherichia coli
U937 Cells
Replicon
Antisense Oligonucleotides
Xenopus
Information Systems
Oocytes
Cytoplasm
Proteins
Experiments
Polymerase Chain Reaction
Messenger RNA

Keywords

  • antisense oligonucleotides
  • MYC
  • RNase H

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of over-expression of bacterial ribonuclease H on the utility of antisense MYC oligodeoxynucleotides in the monocytic leukemia cell line U937. / Rosolen, A.; Kyle, E.; Chavany, C.; Bergan, Raymond; Kalman, E. T.; Crouch, R.; Neckers, L.

In: Biochimie, Vol. 75, No. 1-2, 1993, p. 79-87.

Research output: Contribution to journalArticle

Rosolen, A. ; Kyle, E. ; Chavany, C. ; Bergan, Raymond ; Kalman, E. T. ; Crouch, R. ; Neckers, L. / Effect of over-expression of bacterial ribonuclease H on the utility of antisense MYC oligodeoxynucleotides in the monocytic leukemia cell line U937. In: Biochimie. 1993 ; Vol. 75, No. 1-2. pp. 79-87.
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