Effect of non-nucleoside reverse transcriptase inhibitors on cytokine, chemokine, and immunoglobulin profiles in serum and genital secretions of HIV-infected women

Ravinder Kaur Sachdeva, Ajay Wanchu, Rashmi Bagga, Nancy Malla, Meera Sharma

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Non-protease inhibitor-based antiretroviral therapy (ART) is widely accepted as first-line ART in developing countries. Although reverse transcriptase inhibitor-based regimens have been studied in the peripheral blood, no studies have analyzed alterations in cytokine and chemokine levels, together in peripheral blood and genital secretions. Forty HIV-infected women with CD4 cell counts 3, asymptomatic, with no genital tract infection, willing to participate in the study, and adhere to ART were enrolled. Cervicovaginal lavage (CVL) was collected in the mid-cycle phase of menstrual cycle. Patients were initiated with reverse transcriptase-based antiretrovirals. Repeat sampling was performed at 24 weeks. Cytokines and chemokines were measured using ultrasensitive ELISA kits. Viral load declined to undetectable levels in 29 patients in the blood and in 33 cases in the CVL. Proinflammatory cytokines (tumor necrosis factor-α [TNF-α, interleukin-6 [IL-6], IL-1β) in the serum and CVL showed a significant decrease in mean levels after therapy. IL-2 levels increased significantly whereas IL-12 and (IFN-γ decreased in both compartments. Mean levels of IL-4 and IL-10 decreased significantly in the serum. There was direct correlation between serum and CVL levels of IL-2 and IL-10. IL-10 had a negative correlation with CD4% at baseline and 6 months of therapy. Mean levels of all α- and β-chemokines decreased in serum after therapy. In CVL, mean levels of MIP-1α, RANTES, and IL-8 reduced and SDF-1α increased significantly (P value

Original languageEnglish (US)
Pages (from-to)299-310
Number of pages12
JournalJournal of Interferon and Cytokine Research
Volume30
Issue number5
DOIs
StatePublished - May 1 2010
Externally publishedYes

Fingerprint

Reverse Transcriptase Inhibitors
Chemokines
Therapeutic Irrigation
Immunoglobulins
HIV
Cytokines
Serum
Interleukin-10
Interleukin-2
Therapeutics
Reproductive Tract Infections
Chemokine CCL5
RNA-Directed DNA Polymerase
Interleukin-12
CD4 Lymphocyte Count
Menstrual Cycle
Viral Load
Interleukin-8
Interleukin-1
Interleukin-4

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Cell Biology
  • Medicine(all)

Cite this

Effect of non-nucleoside reverse transcriptase inhibitors on cytokine, chemokine, and immunoglobulin profiles in serum and genital secretions of HIV-infected women. / Sachdeva, Ravinder Kaur; Wanchu, Ajay; Bagga, Rashmi; Malla, Nancy; Sharma, Meera.

In: Journal of Interferon and Cytokine Research, Vol. 30, No. 5, 01.05.2010, p. 299-310.

Research output: Contribution to journalArticle

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