Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats

Toshiaki Nishikawa, Jeffrey Kirsch, Raymond C. Koehler, David S. Bredt, Jeffrey R. Kirsch, Richard J. Traystman

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Background and Purpose: We tested the hypothesis that inhibition of nitric oxide synthase activity in brain before ischemia alters cerebral blood flow and decreases brain injury after 4 hours of middle cerebral artery occlusion in cats. Methods: Halothane-anesthetized cats underwent 4 hours of left middle cerebral artery occlusion after they were randomly assigned to receive either intravenous Nω-nitro-L-arginine methyl ester, at a dose that completely inhibited cortical nitric oxide synthase activity (10 mg/kg, n=10), or an equal volume of diluent (10 mL saline, n=10). Serial blood flow measurements were made with radiolabeled microspheres, and injury volume was measured by triphenyltetrazolium staining. Results: Blood flow to caudate nucleus and inferior temporal cortex decreased to the same extent in both groups during middle cerebral artery occlusion. Somatosensory evoked potential amplitude was reduced to less than 10% of baseline values in both groups. Injury volume of ipsilateral caudate nucleus in cats pretreated with nitroarginine (52±5%, mean±SE) was less (P

Original languageEnglish (US)
Pages (from-to)1717-1724
Number of pages8
JournalStroke
Volume24
Issue number11
StatePublished - 1993
Externally publishedYes

Fingerprint

Cerebrovascular Circulation
Middle Cerebral Artery Infarction
Nitric Oxide Synthase
Cats
Ischemia
Caudate Nucleus
Wounds and Injuries
Somatosensory Evoked Potentials
Nitroarginine
Halothane
Temporal Lobe
Brain Ischemia
Microspheres
Brain Injuries
Staining and Labeling

Keywords

  • Cats
  • Cerebral arteries
  • Cerebral blood flow
  • Evoked potentials
  • Nitric oxide
  • Somatosensory

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Neuroscience(all)

Cite this

Nishikawa, T., Kirsch, J., Koehler, R. C., Bredt, D. S., Kirsch, J. R., & Traystman, R. J. (1993). Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats. Stroke, 24(11), 1717-1724.

Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats. / Nishikawa, Toshiaki; Kirsch, Jeffrey; Koehler, Raymond C.; Bredt, David S.; Kirsch, Jeffrey R.; Traystman, Richard J.

In: Stroke, Vol. 24, No. 11, 1993, p. 1717-1724.

Research output: Contribution to journalArticle

Nishikawa, T, Kirsch, J, Koehler, RC, Bredt, DS, Kirsch, JR & Traystman, RJ 1993, 'Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats', Stroke, vol. 24, no. 11, pp. 1717-1724.
Nishikawa T, Kirsch J, Koehler RC, Bredt DS, Kirsch JR, Traystman RJ. Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats. Stroke. 1993;24(11):1717-1724.
Nishikawa, Toshiaki ; Kirsch, Jeffrey ; Koehler, Raymond C. ; Bredt, David S. ; Kirsch, Jeffrey R. ; Traystman, Richard J. / Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats. In: Stroke. 1993 ; Vol. 24, No. 11. pp. 1717-1724.
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