Effect of nitric oxide synthase inhibition on cerebral blood flow and injury volume during focal ischemia in cats

Toshiaki Nishikawa, Jeffrey R. Kirsch, Raymond C. Koehler, David S. Bredt, Jeffrey R. Kirsch, Richard J. Traystman

Research output: Contribution to journalArticle

106 Scopus citations


Background and Purpose: We tested the hypothesis that inhibition of nitric oxide synthase activity in brain before ischemia alters cerebral blood flow and decreases brain injury after 4 hours of middle cerebral artery occlusion in cats. Methods: Halothane-anesthetized cats underwent 4 hours of left middle cerebral artery occlusion after they were randomly assigned to receive either intravenous Nω-nitro-L-arginine methyl ester, at a dose that completely inhibited cortical nitric oxide synthase activity (10 mg/kg, n=10), or an equal volume of diluent (10 mL saline, n=10). Serial blood flow measurements were made with radiolabeled microspheres, and injury volume was measured by triphenyltetrazolium staining. Results: Blood flow to caudate nucleus and inferior temporal cortex decreased to the same extent in both groups during middle cerebral artery occlusion. Somatosensory evoked potential amplitude was reduced to less than 10% of baseline values in both groups. Injury volume of ipsilateral caudate nucleus in cats pretreated with nitroarginine (52±5%, mean±SE) was less (P<.05) compared with the saline group (80 ±4%), whereas ipsilateral cerebral hemispheric injury volume was similar between the two groups (30±6% and 32±4% of hemisphere in saline and nitroarginine groups, respectively). Conclusions: These results suggest that inhibition of nitric oxide synthase decreases caudate injury volume at 4 hours of middle cerebral artery occlusion without an alteration in distribution of cerebral blood flow.

Original languageEnglish (US)
Pages (from-to)1717-1724
Number of pages8
Issue number11
StatePublished - Nov 1993



  • Cats
  • Cerebral arteries
  • Cerebral blood flow
  • Evoked potentials
  • Nitric oxide
  • Somatosensory

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

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