Effect of liquid diet on nude mouse gastrointestinal tract

F. E. Johnson, E. M. Awad, M. C. Laregina, Clifford Deveney, C. W. Deveney

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The nude (athymic) mouse is currently used to study the effect of gastrin on cancer xenografts. We sought to develop a hypogastrinemic nude mouse model for use in evaluating the effect of hypogastrinemia on such xenografts. Thirty-six non-tumor-bearing nude mice were studied. Eighteen received a nutritionally complete liquid diet; eighteen received standard chow. Six mice from each group were weighed and killed (nonfasting) on days 2, 8, and 15. Mean serum gastrin levels (±SEM) for the control group were 118.7 ± 7.5, 118.7 ± 8.7, and 118.0 ± 7.5 pg/ml on days 2, 8, and 15, respectively. Serum gastrin levels for the liquid diet group significantly decreased 87.0 ± 7.6, 88.0 ± 9.7, and 66.7 ± 9.6 pg/ml on the same days. Animals in both groups gained weight normally; there were no significant weight differences between the two groups at any point. No histological abnormalities were seen in stomach, small intestine, colon, cecum, liver, pancreas, spleen or kidney. However, the liquid diet group showed atrophic changes in colon: significant reductions in colon weight and RNA content on days 8 and 15, and significant reduction in colon protein content on day 8. This model of hypogastrinemia is reliable and inexpensive. The nonsurgical nature of the preparation allows excellent survival in this immunodeficient animal.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalJournal of Surgical Oncology
Volume32
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Nude Mice
Gastrointestinal Tract
Gastrins
Colon
Diet
Heterografts
Weights and Measures
Cecum
Serum
Small Intestine
Pancreas
Stomach
Spleen
RNA
Kidney
Control Groups
Liver
Neoplasms
Proteins

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Johnson, F. E., Awad, E. M., Laregina, M. C., Deveney, C., & Deveney, C. W. (1986). Effect of liquid diet on nude mouse gastrointestinal tract. Journal of Surgical Oncology, 32(1), 50-54.

Effect of liquid diet on nude mouse gastrointestinal tract. / Johnson, F. E.; Awad, E. M.; Laregina, M. C.; Deveney, Clifford; Deveney, C. W.

In: Journal of Surgical Oncology, Vol. 32, No. 1, 1986, p. 50-54.

Research output: Contribution to journalArticle

Johnson, FE, Awad, EM, Laregina, MC, Deveney, C & Deveney, CW 1986, 'Effect of liquid diet on nude mouse gastrointestinal tract', Journal of Surgical Oncology, vol. 32, no. 1, pp. 50-54.
Johnson FE, Awad EM, Laregina MC, Deveney C, Deveney CW. Effect of liquid diet on nude mouse gastrointestinal tract. Journal of Surgical Oncology. 1986;32(1):50-54.
Johnson, F. E. ; Awad, E. M. ; Laregina, M. C. ; Deveney, Clifford ; Deveney, C. W. / Effect of liquid diet on nude mouse gastrointestinal tract. In: Journal of Surgical Oncology. 1986 ; Vol. 32, No. 1. pp. 50-54.
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