Effect of l-glutamate on the release of striatal dopamine: in vivo dialysis and electrochemical studies

Bita Moghaddam, Rand J. Gruen, Robert H. Roth, Benjamin S. Bunney, Ralph N. Adams

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Microdialysis and in vivo voltammetry combined K+-selective microelectrodes were utilized to study the effect of l-glutamate (GLU) on the in vivo release of dopamine (DA) from the rat striatum. Perfusion of 500 nM-5mM GLU through the microdialysis probe was without an effect on DA outflow whereas 10 mM GLU resulted in a significant (295%) increase in the basal level of DA. This increase was blocked in the presence of 2-amino-5-phosphonopentanoic acid, an N-Methyl-d-aspartate (NMDA) receptor antagonist. Repetitive local applications of 10 mM GLU were also required to observe an increase in extracellular DA measured by in vivo voltammetry. These signals were accompanied with a massive increase in extracellular K+ and a large negative shift in the field potential resembling the ionic changes seen after the phenomenon spreading depression. These studies suggest that high concentrations of GLU are required to enhance the extracellular concentration of DA in vivo. Further, pathophysiological conditions such as spreading depression may be responsible for the observed increase in extracellular DA concentration.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalBrain Research
Volume518
Issue number1-2
DOIs
StatePublished - Jun 4 1990
Externally publishedYes

Fingerprint

Corpus Striatum
Dialysis
Glutamic Acid
Dopamine
Microdialysis
2-Amino-5-phosphonovalerate
Microelectrodes
Perfusion

Keywords

  • Dopamine
  • Glutamate
  • In vivo voltammetry
  • Microdialysis
  • N-Methyl-d-aspartate receptor
  • Potassium
  • Striatum

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Effect of l-glutamate on the release of striatal dopamine : in vivo dialysis and electrochemical studies. / Moghaddam, Bita; Gruen, Rand J.; Roth, Robert H.; Bunney, Benjamin S.; Adams, Ralph N.

In: Brain Research, Vol. 518, No. 1-2, 04.06.1990, p. 55-60.

Research output: Contribution to journalArticle

Moghaddam, Bita ; Gruen, Rand J. ; Roth, Robert H. ; Bunney, Benjamin S. ; Adams, Ralph N. / Effect of l-glutamate on the release of striatal dopamine : in vivo dialysis and electrochemical studies. In: Brain Research. 1990 ; Vol. 518, No. 1-2. pp. 55-60.
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