Effect of Ionizing Radiation on the Physical Biology of Head and Neck Squamous Cell Carcinoma Cells

Sandra M. Baker-Groberg, Sophia Bornstein, Jevgenia Zilberman-Rudenko, Mark Schmidt, Garth W. Tormoen, Casey Kernan, Charles Thomas, Melissa Wong, Kevin G. Phillips, Owen McCarty

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Head and neck squamous cell carcinoma (HNSCC) is the sixth leading cause of cancer worldwide. Although there are numerous treatment options for HNSCC, such as surgery, cytotoxic chemotherapy, molecularly targeted systemic therapeutics, and radiotherapy, overall survival has not significantly improved in the last 50 years. This suggests a need for a better understanding of how these cancer cells respond to current treatments in order to improve treatment paradigms. Ionizing radiation (IR) promotes cancer cell death through the creation of cytotoxic DNA lesions, including single strand breaks, base damage, crosslinks, and double strand breaks (DSBs). As unrepaired DSBs are the most cytotoxic DNA lesion, defining the downstream cellular responses to DSBs are critical for understanding the mechanisms of tumor cell responses to IR. The effects of experimental IR on HNSCC cells beyond DNA damage in vitro are ill-defined. Here we combined label-free, quantitative phase and fluorescent microscopy to define the effects of IR on the dry mass and volume of the HNSCC cell line, UM-SCC-22A. We quantified nuclear and cytoplasmic subcellular density alterations resulting from 8 Gy X-ray IR and correlated these signatures with DNA and γ-H2AX expression patterns. This study utilizes a synergistic imaging approach to study both biophysical and biochemical alterations in cells following radiation damage and will aid in future understanding of cellular responses to radiation therapy.

Original languageEnglish (US)
Pages (from-to)517-525
Number of pages9
JournalCellular and Molecular Bioengineering
Volume8
Issue number3
DOIs
StatePublished - Sep 21 2015

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Ionizing radiation
Ionizing Radiation
Biology
Radiation
Cells
DNA
Cell
Radiotherapy
Neoplasms
Cancer
Chemotherapy
Radiation damage
Cell death
Therapeutics
Surgery
DNA Damage
Labels
Tumors
Microscopy
Microscopic examination

Keywords

  • Head and neck squamous cell carcinoma
  • Physical biology
  • Quantitative phase microscopy
  • Radiation damage

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

Cite this

Effect of Ionizing Radiation on the Physical Biology of Head and Neck Squamous Cell Carcinoma Cells. / Baker-Groberg, Sandra M.; Bornstein, Sophia; Zilberman-Rudenko, Jevgenia; Schmidt, Mark; Tormoen, Garth W.; Kernan, Casey; Thomas, Charles; Wong, Melissa; Phillips, Kevin G.; McCarty, Owen.

In: Cellular and Molecular Bioengineering, Vol. 8, No. 3, 21.09.2015, p. 517-525.

Research output: Contribution to journalArticle

Baker-Groberg, SM, Bornstein, S, Zilberman-Rudenko, J, Schmidt, M, Tormoen, GW, Kernan, C, Thomas, C, Wong, M, Phillips, KG & McCarty, O 2015, 'Effect of Ionizing Radiation on the Physical Biology of Head and Neck Squamous Cell Carcinoma Cells', Cellular and Molecular Bioengineering, vol. 8, no. 3, pp. 517-525. https://doi.org/10.1007/s12195-015-0393-8
Baker-Groberg SM, Bornstein S, Zilberman-Rudenko J, Schmidt M, Tormoen GW, Kernan C et al. Effect of Ionizing Radiation on the Physical Biology of Head and Neck Squamous Cell Carcinoma Cells. Cellular and Molecular Bioengineering. 2015 Sep 21;8(3):517-525. https://doi.org/10.1007/s12195-015-0393-8
Baker-Groberg, Sandra M. ; Bornstein, Sophia ; Zilberman-Rudenko, Jevgenia ; Schmidt, Mark ; Tormoen, Garth W. ; Kernan, Casey ; Thomas, Charles ; Wong, Melissa ; Phillips, Kevin G. ; McCarty, Owen. / Effect of Ionizing Radiation on the Physical Biology of Head and Neck Squamous Cell Carcinoma Cells. In: Cellular and Molecular Bioengineering. 2015 ; Vol. 8, No. 3. pp. 517-525.
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