Effect of Increasing Maternal Body Mass Index on Oxidative and Nitrative Stress in The Human Placenta

Victoria Roberts, J. Smith, S. A. McLea, A. B. Heizer, J. L. Richardson, Leslie Myatt

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Maternal obesity is an increasing problem in obstetrics associated with adverse pregnancy outcomes and delivery complications. As an inflammatory state, where elevated levels of pro-inflammatory cytokines are found, obesity can lead to the increased incidence of oxidative and nitrative stress. These stresses may result in protein oxidation and protein nitration respectively, which are post- translational covalent modifications that can modify the structure and subsequently alter the function of a protein. The objective of this study was to examine whether placental oxidative and nitrative stress increase with increasing maternal body mass index. Placental tissue was collected from three groups of patients categorized as lean, overweight and obese. The presence of nitrotyrosine residues, a marker of nitrative stress, and antioxidant enzymes, as markers of oxidative stress, were assessed by immunohistochemistry, Western blot and ELISA. Protein carbonyl formation, a specific measure of protein oxidation, was measured by OxyBlot™ kit. Nitrotyrosine residues were increased in obese compared to lean and overweight groups although localization was unaltered across the three groups. Superoxide dismutase enzyme expression, localization and activity was unaltered between the groups. Protein carbonyl formation was greater in the lean compared to the overweight individuals. This study demonstrates that with increasing maternal body mass index there is an increase in placental nitrative stress. There does not appear to be a corresponding increase in oxidative stress and indeed we demonstrate some evidence of a decrease in oxidative effects in these placenta samples. Potentially the formation of peroxynitrite may be consuming reactive oxygen species and reducing oxidative stress. There may be a shift in the balance between nitrative and oxidative stress, which may be a protective mechanism for the placenta.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalPlacenta
Volume30
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Placenta
Oxidative Stress
Body Mass Index
Mothers
Protein Carbonylation
Proteins
Obesity
Peroxynitrous Acid
Enzymes
Pregnancy Outcome
Post Translational Protein Processing
Obstetrics
Superoxide Dismutase
Reactive Oxygen Species
Antioxidants
Western Blotting
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Cytokines
Incidence

Keywords

  • Nitrative stress
  • Obesity
  • Oxidative stress

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Effect of Increasing Maternal Body Mass Index on Oxidative and Nitrative Stress in The Human Placenta. / Roberts, Victoria; Smith, J.; McLea, S. A.; Heizer, A. B.; Richardson, J. L.; Myatt, Leslie.

In: Placenta, Vol. 30, No. 2, 02.2009, p. 169-175.

Research output: Contribution to journalArticle

Roberts, Victoria ; Smith, J. ; McLea, S. A. ; Heizer, A. B. ; Richardson, J. L. ; Myatt, Leslie. / Effect of Increasing Maternal Body Mass Index on Oxidative and Nitrative Stress in The Human Placenta. In: Placenta. 2009 ; Vol. 30, No. 2. pp. 169-175.
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