Effect of increased intraoctlar pressure on retinal ganglion cells morphology in albino rat

F. Ahmed, P. Chaudhary, S. C. Sharma

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Abstract

Purpose, To analyze the effecls of elevated intraocular pressure (IOP) on retinal ganglion cells (RGC's) morphology in rats. Methods. In the adult wistar albino rats, IOP was elevaied in one eye by cauterizing two Hmbal derived épi scierai vein under deep anesthesia. Contralateral eye served as a control for each animal. Elevated pressure was maintained uplo 11 weeks which ranged between 1.5 - 1.8 time higher w hen compared to contralateral control eye. RGC's of both eyes were retrogradely labeled with contralateral tectal injections of 5% Fluoro-Gold. RGC's of both eyes were counted in flat mounted retinae. RGC's Sonia diameter of normal eyes were compared with those of elevated IOP eyes . Results. In normal eyes the mean soma size for the small, medium and large types of RGC's were 7.5. 12 and 16.5 um. respectively. In the experimental eyes with elevated IOP. the mean soma size of small, medium and large lypes of cells were 9.57, 13 and 18.61 [Am. respectively. Conclusions. The RGC's death was 42-48% afler 11 weeks of elevaied IOP. At the same time remaining RGC's diameter increased in all cell lypes. respectively. We believe lhat there may be corresponding increase in the dendritic field of Ine remaining cells. Such studies are presently under itivestigalion.Supported by N.I.H., 5ROIEY11205.

Original languageEnglish (US)
Pages (from-to)S160
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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