Effect of hyperinsulinaemia on renal function and nitrate/nitrite excretion in healthy subjects

Radko Komers, Terezie Pelikanová, Ludmila Kazdová

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

1. In addition to its metabolic actions, insulin acts as a vasodilator in certain vascular beds, such as skeletal muscle. It has been shown that this effect is mediated by endothelium-derived nitric oxide (NO). Unlike in the skeletal muscle, insulin-NO interactions in the kidney, another major site of insulin action, have been less studied. The aim of the present study was to explore the role of NO in renal effects of hyperinsulinaemia in healthy subjects. 2. Changes in renal function and urinary nitrate/nitrite (NO2 -/NO3 -; Griess method) levels as a marker of renal production of NO were assessed during euglycaemic hyperinsulinaemic clamp and compared with normoinsulinaemic isovolaemic conditions (administration of the same amount of insulin/glucose-free vehicle) in 10 healthy male volunteers. 3. Hyperinsulinaemia was associated with a decrease in renal excretion of stable metabolites of NO (mean (±SEM) 0.56±0.12 vs 0.38±0.05 μmol/min, respectively; P2 -/NO3 - (P2 -/NO3 - with a significantly different response to hyperinsulinaemia when compared with normoinsulinaemia (F = 12.2; P

Original languageEnglish (US)
Pages (from-to)336-341
Number of pages6
JournalClinical and Experimental Pharmacology and Physiology
Volume26
Issue number4
DOIs
StatePublished - 1999

Fingerprint

Hyperinsulinism
Nitrites
Nitrates
Healthy Volunteers
Nitric Oxide
Kidney
Insulin
Skeletal Muscle
Glucose Clamp Technique
Vasodilator Agents
Blood Vessels
Glucose

Keywords

  • Hyperinsulinaemia
  • Natriuresis
  • Nitrate/nitrite
  • Nitric oxide
  • Renal

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effect of hyperinsulinaemia on renal function and nitrate/nitrite excretion in healthy subjects. / Komers, Radko; Pelikanová, Terezie; Kazdová, Ludmila.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 26, No. 4, 1999, p. 336-341.

Research output: Contribution to journalArticle

Komers, Radko ; Pelikanová, Terezie ; Kazdová, Ludmila. / Effect of hyperinsulinaemia on renal function and nitrate/nitrite excretion in healthy subjects. In: Clinical and Experimental Pharmacology and Physiology. 1999 ; Vol. 26, No. 4. pp. 336-341.
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