Effect of granulocyte-macrophage colony-stimulating factor and interleukin-3 on interleukin-8 production by human neutrophils and monocytes

Gary Takahashi, D. Frank Andrews, Michael B. Lilly, Jack W. Singer, Mark R. Alderson

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Interleukin-8 (IL-8) is a major neutrophil chemoattractant and functional stimulant that is induced by IL-1, tumor necrosis factor alpha (TNFα), and lipopolysaccharide (LPS). We report that recombinant human (rh) granulocyte-macrophage colony-stimulating factor (GM-CSF) and rhIL-3 are also potent inducers of IL-8 messenger RNA (mRNA) accumulation and protein secretion by normal peripheral blood monocytes. Neutrophils produce IL-8 in response to GM-CSF but not to IL-3. In contrast, recombinant human granulocyte-CSF (rhG-CSF), at concentrations as high as 100 ng/mL, does not induce IL-8 in either cell type. rhGM-CSF also induces IL-8 mRNA expression and IL-8 protein in the promonocytic cell line, U-937, whereas rhG-CSF does not. IL-8 secretion by monocytes was stimulated within 2 hours after incubation with rhGM-CSF or rhIL-3. Stimulation of neutrophils with rhGM-CSF resulted in an increase in cell-associated IL-8 at 4 hours. At 24 hours, cell-associated IL-8 levels declined, whereas secreted IL-8 levels increased. In contrast, virtually all IL-8 induced in monocytes appeared as secreted protein. Neither rhGM-CSF nor rhIL-3 induced detectable secretion of IL-1, TNFα, or IL-6 protein by monocytes. rhGM-CSF, and to a lesser degree rhIL-3, potently stimulated IL-8 secretion in cultures of heparinized whole blood, whereas rhG-CSF had no significant effect on IL-8 secretion. Induction of IL-8 by GM-CSF may be physiologically important in enhancing the acute inflammatory response.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalBlood
Volume81
Issue number2
StatePublished - Jan 15 1993
Externally publishedYes

Fingerprint

Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Monocytes
Neutrophils
Granulocytes
Interleukin-1
Proteins
Blood
Tumor Necrosis Factor-alpha
Messenger RNA
Chemotactic Factors
Lipopolysaccharides
Interleukin-6

ASJC Scopus subject areas

  • Hematology

Cite this

Effect of granulocyte-macrophage colony-stimulating factor and interleukin-3 on interleukin-8 production by human neutrophils and monocytes. / Takahashi, Gary; Andrews, D. Frank; Lilly, Michael B.; Singer, Jack W.; Alderson, Mark R.

In: Blood, Vol. 81, No. 2, 15.01.1993, p. 357-364.

Research output: Contribution to journalArticle

Takahashi, Gary ; Andrews, D. Frank ; Lilly, Michael B. ; Singer, Jack W. ; Alderson, Mark R. / Effect of granulocyte-macrophage colony-stimulating factor and interleukin-3 on interleukin-8 production by human neutrophils and monocytes. In: Blood. 1993 ; Vol. 81, No. 2. pp. 357-364.
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