Effect of genetic cross on the detection of quantitative trait loci and a novel approach to mapping QTLs

Robert Hitzemann, Kristin Demarest, Jay Koyner, Laura Cipp, Nilay Patel, Eric Rasmussen, James McCaughran

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A genome-wide scan was conducted in two F2 intercrosses, C57BL/6J (B6) × DBA/2J (D2) and BALB/cJ (C) × LP/J (LP), for three different phenotypes: basal locomotor activity, ethanol-induced locomotor activity, and haloperidol-induced catalepsy. For basal activity, significant quantitative trait loci (QTLs, LOD ≥ 4.3) were detected on chromosomes 9 and 19 for the C × LP intercross and chromosome 1 for the B6 × D2 intercross. Significant QTLs for ethanol-induced activation were detected on chromosome 6 for the C × LP intercross, and on chromosomes 1 and 2 for the B6 × D2 intercross. For haloperidol-induced catalepsy, significant QTLs were detected on chromosome 14 (two different QTLs) in the C × LP intercross, and chromosomes 1 and 9 in the B6 × D2 intercross. These data illustrate the importance of the genetic cross for QTL detection. Finally, the data reported here, and elsewhere, are also used to demonstrate a novel approach to QTL detection and localization.

Original languageEnglish (US)
Pages (from-to)767-772
Number of pages6
JournalPharmacology Biochemistry and Behavior
Volume67
Issue number4
DOIs
StatePublished - 2000

Fingerprint

Genetic Crosses
Chromosomes, Human, Pair 1
Quantitative Trait Loci
Chromosomes
Catalepsy
Chromosomes, Human, Pair 9
Haloperidol
Locomotion
Ethanol
Chromosomes, Human, 6-12 and X
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 2
Genome
Phenotype
Genes
Chemical activation

Keywords

  • Catalepsy
  • Ethanol
  • Haloperidol
  • Locomotor activity
  • Mouse
  • Quantitative trait loci

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Effect of genetic cross on the detection of quantitative trait loci and a novel approach to mapping QTLs. / Hitzemann, Robert; Demarest, Kristin; Koyner, Jay; Cipp, Laura; Patel, Nilay; Rasmussen, Eric; McCaughran, James.

In: Pharmacology Biochemistry and Behavior, Vol. 67, No. 4, 2000, p. 767-772.

Research output: Contribution to journalArticle

Hitzemann, Robert ; Demarest, Kristin ; Koyner, Jay ; Cipp, Laura ; Patel, Nilay ; Rasmussen, Eric ; McCaughran, James. / Effect of genetic cross on the detection of quantitative trait loci and a novel approach to mapping QTLs. In: Pharmacology Biochemistry and Behavior. 2000 ; Vol. 67, No. 4. pp. 767-772.
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