Effect of estradiol-17β on preprolactin messenger ribonucleic acid activity in the rat pituitary gland

Roger T. Stone, Richard A. Maurer, Richard Maurer

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Rat pituitary RNA was translated in the wheat germ system. Preprolactin messenger RNA activity was estimated by adsorption of cell-free products to solid phase antiprolactin. When male rats were injected for 4 days with estradiol-17β, pituitary preprolactin mRNA activity was increased 2.5- to 3.0-fold over controls. This increase was evident when either total RNA, poly(adenylic acid) RNA, or polysomal RNA was translated in the cell-free system. In male rats receiving daily injections of estradiol-17β, preprolactin mRNA activity was increased to an apparent maximum of 300% of controls after 7 days of treatment. Our data also indicate that estradiol increases preprolactin mRNA activity per μg of RNA as well as the pituitary content of RNA. After estradiol treatment was discontinued, preprolactin mRNA activity declined to 50% of the maximum stimulation after approximately 2 days. In ovariectomized retired breeder female rats, a 5-fold increase in preprolactin activity over ovariectomized controls was obtained. In other studies, a 2-fold increase in preprolactin mRNA activity was obtained in male rats 24 h after a single injection of pimozide, a dopamine blocking drug.

Original languageEnglish (US)
Pages (from-to)4915-4921
Number of pages7
JournalBiochemistry
Volume16
Issue number22
StatePublished - 1977
Externally publishedYes

Fingerprint

Pituitary Gland
Rats
Estradiol
RNA
Messenger RNA
Pimozide
Dopamine Agents
Injections
Cell-Free System
Adenosine Monophosphate
preprolactin
Adsorption
Triticum
Dopamine
Therapeutics
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of estradiol-17β on preprolactin messenger ribonucleic acid activity in the rat pituitary gland. / Stone, Roger T.; Maurer, Richard A.; Maurer, Richard.

In: Biochemistry, Vol. 16, No. 22, 1977, p. 4915-4921.

Research output: Contribution to journalArticle

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