Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermisstudied by reflectance confocal microscopy

Steven Jacques, R. Samatham, K. G. Phillips

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Optical clearing of mouse dermis by glycerol was tested by reflectance-mode confocal microscopy (rCSLM) using 488- nm light. The reflectance signal R(z) was acquired as a function of the depth of the focus (z) within the upper 100 μm of freshly excised mouse dermis. The results specify the scattering coefficient (μs [cm-1]) and the anisotropy of scattering (g [dimensionless]). The absorption is too low to exert an effect. The results, published in Samatham et al., Journal of Innovative Optical Health Sciences 2010, 3(3):183-188, described how the clearing effect of glycerol was to increase g toward nearly 1.0, while having only a modest effect on μs. In other words, glycerol caused light scattering to become very forward-directed, but did not strongly alter the number of scattering events per unit length of photon path. This paper discusses the possible mechanism of action that is responsible for this clearing effect.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7907
DOIs
StatePublished - 2011
EventBiomedical Applications of Light Scattering V - San Francisco, CA, United States
Duration: Jan 22 2011Jan 24 2011

Other

OtherBiomedical Applications of Light Scattering V
CountryUnited States
CitySan Francisco, CA
Period1/22/111/24/11

Fingerprint

clearing
Confocal microscopy
Anisotropy
scattering coefficients
Glycerol
Confocal Microscopy
glycerols
Scattering
Dermis
microscopy
reflectance
anisotropy
scattering
mice
Light
Laser modes
Photons
Light scattering
Health
health

Keywords

  • Biomedical optics
  • Collagen
  • Optical clearing
  • Scattering
  • Skin

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Jacques, S., Samatham, R., & Phillips, K. G. (2011). Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermisstudied by reflectance confocal microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7907). [790706] https://doi.org/10.1117/12.874060

Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermisstudied by reflectance confocal microscopy. / Jacques, Steven; Samatham, R.; Phillips, K. G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7907 2011. 790706.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jacques, S, Samatham, R & Phillips, KG 2011, Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermisstudied by reflectance confocal microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7907, 790706, Biomedical Applications of Light Scattering V, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.874060
Jacques S, Samatham R, Phillips KG. Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermisstudied by reflectance confocal microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7907. 2011. 790706 https://doi.org/10.1117/12.874060
Jacques, Steven ; Samatham, R. ; Phillips, K. G. / Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermisstudied by reflectance confocal microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7907 2011.
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