Effect of carotenoid supplemented formula on carotenoid bioaccumulation in tissues of infant rhesus macaques: A pilot study focused on lutein

Sookyoung Jeon, Martha Neuringer, Emily E. Johnson, Matthew J. Kuchan, Suzette L. Pereira, Elizabeth J. Johnson, John W. Erdman

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Lutein is the predominant carotenoid in the developing primate brain and retina, and may have important functional roles. However, its bioaccumulation pattern during early development is not understood. In this pilot study, we investigated whether carotenoid supplementation of infant formula enhanced lutein tissue deposition in infant rhesus macaques. Monkeys were initially breastfed; from 1 to 3 months of age they were fed either a formula supplemented with lutein, zeaxanthin, β-carotene and lycopene, or a control formula with low levels of these carotenoids, for 4 months (n = 2/group). All samples were analyzed by high pressure liquid chromatography (HPLC). Final serum lutein in the supplemented group was 5 times higher than in the unsupplemented group. All brain regions examined showed a selective increase in lutein deposition in the supplemented infants. Lutein differentially accumulated across brain regions, with highest amounts in occipital cortex in both groups. β-carotene accumulated, but zeaxanthin and lycopene were undetectable in any brain region. Supplemented infants had higher lutein concentrations in peripheral retina but not in macular retina. Among adipose sites, abdominal subcutaneous adipose tissue exhibited the highest lutein level and was 3-fold higher in the supplemented infants. The supplemented formula enhanced carotenoid deposition in several other tissues. In rhesus infants, increased intake of carotenoids from formula enhanced their deposition in serum and numerous tissues and selectively increased lutein in multiple brain regions.

    Original languageEnglish (US)
    Article number51
    JournalNutrients
    Volume9
    Issue number1
    DOIs
    StatePublished - Jan 10 2017

    Fingerprint

    Lutein
    bioaccumulation
    Carotenoids
    Macaca mulatta
    lutein
    carotenoids
    brain
    retina
    Brain
    Retina
    zeaxanthin
    carotenes
    lycopene
    Abdominal Subcutaneous Fat
    tissues
    Occipital Lobe
    Infant Formula
    feed formulation
    infant formulas
    Serum

    Keywords

    • Bioaccumulation
    • Brain
    • Carotenoids
    • Formula
    • Infants
    • Lutein
    • Retina
    • Rhesus macaques

    ASJC Scopus subject areas

    • Food Science

    Cite this

    Effect of carotenoid supplemented formula on carotenoid bioaccumulation in tissues of infant rhesus macaques : A pilot study focused on lutein. / Jeon, Sookyoung; Neuringer, Martha; Johnson, Emily E.; Kuchan, Matthew J.; Pereira, Suzette L.; Johnson, Elizabeth J.; Erdman, John W.

    In: Nutrients, Vol. 9, No. 1, 51, 10.01.2017.

    Research output: Contribution to journalArticle

    Jeon, Sookyoung ; Neuringer, Martha ; Johnson, Emily E. ; Kuchan, Matthew J. ; Pereira, Suzette L. ; Johnson, Elizabeth J. ; Erdman, John W. / Effect of carotenoid supplemented formula on carotenoid bioaccumulation in tissues of infant rhesus macaques : A pilot study focused on lutein. In: Nutrients. 2017 ; Vol. 9, No. 1.
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