Effect of caloric restriction on base-excision repair (BER) in the aging rat brain

Glen E. Kisby, Steven Kohama, Antoinette Olivas, Mona Churchwell, Daniel Doerge, Edward Spangler, Rafael de Cabo, Donald K. Ingram, Barry Imhof, Gaobin Bao, Yoke W. Kow

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Apyrimidinic/apurinic endonuclease (APE) is a key protein involved in the base-excision DNA repair (BER) pathway of oxidative DNA lesions. Using a novel oligonucleotide substrate, we demonstrate that APE activity in the frontal/parietal cortex (F/PCTX), cerebellum, brainstem, midbrain and hypothalamus declined with age in rats on an ad libitum (AL) diet. In contrast, APE activity for these brain regions was ∼1.5-3 times higher in young, caloric restricted (CR) rats. Despite continuous CR treatment in all animals since six weeks of age, APE activity in the CR group started to decline by middle-age and continued into old age. However, CR maintained APE activity at a level that was significantly higher than that in AL rats across age and in the brain regions examined. Because Western analysis of APE, DNA polymerase β and DNA ligase III levels in the F/PCTX of both CR and AL rats remained unchanged with age, this suggests that the increased APE activity in CR rats is the result of differential post-translational modification of APE.

    Original languageEnglish (US)
    Pages (from-to)208-216
    Number of pages9
    JournalExperimental Gerontology
    Volume45
    Issue number3
    DOIs
    StatePublished - Mar 2010

    Fingerprint

    DNA-(Apurinic or Apyrimidinic Site) Lyase
    Caloric Restriction
    DNA Repair
    Rats
    Brain
    Repair
    Aging of materials
    Parietal Lobe
    Frontal Lobe
    DNA Ligases
    DNA
    DNA-Directed DNA Polymerase
    Nutrition
    Oligonucleotides
    Post Translational Protein Processing
    Mesencephalon
    Cerebellum
    Animals
    Hypothalamus
    Brain Stem

    Keywords

    • 8-Oxodeoxyguanosine
    • Apyrimidinic/apurinic endonuclease (APE)
    • DNA ligase III
    • DNA polymerase β
    • Exonuclease
    • Frontal/parietal cortex

    ASJC Scopus subject areas

    • Aging
    • Biochemistry
    • Cell Biology
    • Endocrinology
    • Genetics
    • Molecular Biology

    Cite this

    Kisby, G. E., Kohama, S., Olivas, A., Churchwell, M., Doerge, D., Spangler, E., ... Kow, Y. W. (2010). Effect of caloric restriction on base-excision repair (BER) in the aging rat brain. Experimental Gerontology, 45(3), 208-216. https://doi.org/10.1016/j.exger.2009.12.003

    Effect of caloric restriction on base-excision repair (BER) in the aging rat brain. / Kisby, Glen E.; Kohama, Steven; Olivas, Antoinette; Churchwell, Mona; Doerge, Daniel; Spangler, Edward; Cabo, Rafael de; Ingram, Donald K.; Imhof, Barry; Bao, Gaobin; Kow, Yoke W.

    In: Experimental Gerontology, Vol. 45, No. 3, 03.2010, p. 208-216.

    Research output: Contribution to journalArticle

    Kisby, GE, Kohama, S, Olivas, A, Churchwell, M, Doerge, D, Spangler, E, Cabo, RD, Ingram, DK, Imhof, B, Bao, G & Kow, YW 2010, 'Effect of caloric restriction on base-excision repair (BER) in the aging rat brain', Experimental Gerontology, vol. 45, no. 3, pp. 208-216. https://doi.org/10.1016/j.exger.2009.12.003
    Kisby GE, Kohama S, Olivas A, Churchwell M, Doerge D, Spangler E et al. Effect of caloric restriction on base-excision repair (BER) in the aging rat brain. Experimental Gerontology. 2010 Mar;45(3):208-216. https://doi.org/10.1016/j.exger.2009.12.003
    Kisby, Glen E. ; Kohama, Steven ; Olivas, Antoinette ; Churchwell, Mona ; Doerge, Daniel ; Spangler, Edward ; Cabo, Rafael de ; Ingram, Donald K. ; Imhof, Barry ; Bao, Gaobin ; Kow, Yoke W. / Effect of caloric restriction on base-excision repair (BER) in the aging rat brain. In: Experimental Gerontology. 2010 ; Vol. 45, No. 3. pp. 208-216.
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