Effect of bleomycin on deoxyribonucleic acid synthesis in toluene treated Escherichia coli cells

S. L. Ross, R. E. Moses

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene treated E. coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. These results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.

Original languageEnglish (US)
Pages (from-to)239-246
Number of pages8
JournalAntimicrobial agents and chemotherapy
Volume9
Issue number2
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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