Abstract
The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene treated E. coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. These results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.
Original language | English (US) |
---|---|
Pages (from-to) | 239-246 |
Number of pages | 8 |
Journal | Antimicrobial agents and chemotherapy |
Volume | 9 |
Issue number | 2 |
DOIs | |
State | Published - 1976 |
Externally published | Yes |
ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)
- Infectious Diseases