Effect of anti-IL-15 administration on T Cell and NK cell homeostasis in rhesus macaques

Maren Q. Degottardi, Afam Okoye, Mukta Vaidya, Aarthi Talla, Audrie L. Konfe, Matthew D. Reyes, Joseph A. Clock, Derick M. Duell, Alfred W. Legasse, Amit Sabnis, Byung Park, Michael Axthelm, Jacob Estes, Keith A. Reiman, Rafick Pierre Sekaly, Louis Picker

Research output: Contribution to journalArticle

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Abstract

IL-15 has been implicated as a key regulator of Tand NK cell homeostasis in multiple systems; however, its specific role in maintaining peripheral Tand NK cell populations relative to other γ-chain (γc) cytokines has not been fully defined in primates. In this article, we address this question by determining the effect of IL-15 inhibition with a rhesusized anti-IL-15 mAb on T and NK cell dynamics in rhesus macaques. Strikingly, anti-IL-15 treatment resulted in rapid depletion of NK cells and both CD4+ and CD8+ effector memory T cells (TEM) in blood and tissues, with little to no effect on naive or central memory T cells. Importantly, whereas depletion of NK cells was nearly complete and maintained as long as anti-IL-15 treatment was given, TEM depletion was countered by the onset of massive TEM proliferation, which almost completely restored circulating TEM numbers. Tissue TEM, however, remained significantly reduced, and most TEM maintained very high turnover throughout anti-IL-15 treatment. In the presence of IL-15 inhibition, TEM became increasingly more sensitive to IL-7 stimulation in vivo, and transcriptional analysis of TEM in IL-15-inhibited monkeys revealed engagement of the JAK/STAT signaling pathway, suggesting alternative γc cytokine signaling may support TEM homeostasis in the absence of IL-15. Thus, IL-15 plays a major role in peripheral maintenance of NK cells and TEM. However, whereas most NK cell populations collapse in the absence of IL-15, TEM can be maintained in the face of IL-15 inhibition by the activity of other homeostatic regulators, most likely IL-7.

Original languageEnglish (US)
Pages (from-to)1183-1198
Number of pages16
JournalJournal of Immunology
Volume197
Issue number4
DOIs
StatePublished - Aug 15 2016

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Interleukin-15
Macaca mulatta
Natural Killer Cells
Homeostasis
T-Lymphocytes
Interleukin-7
Cytokines
Primates
Population
Haplorhini

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Effect of anti-IL-15 administration on T Cell and NK cell homeostasis in rhesus macaques. / Degottardi, Maren Q.; Okoye, Afam; Vaidya, Mukta; Talla, Aarthi; Konfe, Audrie L.; Reyes, Matthew D.; Clock, Joseph A.; Duell, Derick M.; Legasse, Alfred W.; Sabnis, Amit; Park, Byung; Axthelm, Michael; Estes, Jacob; Reiman, Keith A.; Sekaly, Rafick Pierre; Picker, Louis.

In: Journal of Immunology, Vol. 197, No. 4, 15.08.2016, p. 1183-1198.

Research output: Contribution to journalArticle

Degottardi, MQ, Okoye, A, Vaidya, M, Talla, A, Konfe, AL, Reyes, MD, Clock, JA, Duell, DM, Legasse, AW, Sabnis, A, Park, B, Axthelm, M, Estes, J, Reiman, KA, Sekaly, RP & Picker, L 2016, 'Effect of anti-IL-15 administration on T Cell and NK cell homeostasis in rhesus macaques', Journal of Immunology, vol. 197, no. 4, pp. 1183-1198. https://doi.org/10.4049/jimmunol.1600065
Degottardi, Maren Q. ; Okoye, Afam ; Vaidya, Mukta ; Talla, Aarthi ; Konfe, Audrie L. ; Reyes, Matthew D. ; Clock, Joseph A. ; Duell, Derick M. ; Legasse, Alfred W. ; Sabnis, Amit ; Park, Byung ; Axthelm, Michael ; Estes, Jacob ; Reiman, Keith A. ; Sekaly, Rafick Pierre ; Picker, Louis. / Effect of anti-IL-15 administration on T Cell and NK cell homeostasis in rhesus macaques. In: Journal of Immunology. 2016 ; Vol. 197, No. 4. pp. 1183-1198.
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AU - Degottardi, Maren Q.

AU - Okoye, Afam

AU - Vaidya, Mukta

AU - Talla, Aarthi

AU - Konfe, Audrie L.

AU - Reyes, Matthew D.

AU - Clock, Joseph A.

AU - Duell, Derick M.

AU - Legasse, Alfred W.

AU - Sabnis, Amit

AU - Park, Byung

AU - Axthelm, Michael

AU - Estes, Jacob

AU - Reiman, Keith A.

AU - Sekaly, Rafick Pierre

AU - Picker, Louis

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