Effect of anemia on the fasciocutaneous flap survival in a rat model

S. Desyatnikova, C. Winslow, James Cohen, Mark Wax

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective/Hypothesis: Ablative surgery for head and neck cancer that creates large composite defects often results in a significant decrease in the hematocrit level. These defects are best reconstructed with a microvascular free tissue transfer. Effect of the decreased hematocrit on microvascular flap survival is controversial. The purpose of this study was to assess the effect of isovolemic anemia on flap survival in a rat model. Study Design: Prospective analysis. Methods: Ninety rats were used (30 control and 60 experimental animals). Experimental animals were rendered anemic by blood draw and volume resuscitated with either a colloid (30 animals) or crystalloid (30 animals) solution. In all animals a ventral fasciocutaneous flap was raised. A vascular clamp was applied to the arteriovenous pedicle, and different ischemic times were allowed to elapse before clamp removal. Flap survival was assessed at 5 days. Probit analysis was performed for the three animal groups. Results: A significantly increased probability of the flap survival was found in the anemic animals compared with the control group (P ≤.05). No difference was found between the colloid and crystalloid resuscitation groups. Conclusions: A decreased hematocrit level increases fasciocutaneous flap tolerance to ischemia and significantly increases the primary ischemic time in the ventral flap clamp model in rats. Fluid replacement with either crystalloid or colloid produces identical results.

Original languageEnglish (US)
Pages (from-to)572-575
Number of pages4
JournalLaryngoscope
Volume111
Issue number4 I
StatePublished - 2001

Fingerprint

Anemia
Colloids
Hematocrit
Head and Neck Neoplasms
Blood Volume
Resuscitation
Blood Vessels
Ischemia
Prospective Studies
Control Groups
crystalloid solutions

Keywords

  • Anemia
  • Animal model
  • Free tissue transfer
  • Hematocrit

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Effect of anemia on the fasciocutaneous flap survival in a rat model. / Desyatnikova, S.; Winslow, C.; Cohen, James; Wax, Mark.

In: Laryngoscope, Vol. 111, No. 4 I, 2001, p. 572-575.

Research output: Contribution to journalArticle

Desyatnikova, S, Winslow, C, Cohen, J & Wax, M 2001, 'Effect of anemia on the fasciocutaneous flap survival in a rat model', Laryngoscope, vol. 111, no. 4 I, pp. 572-575.
Desyatnikova, S. ; Winslow, C. ; Cohen, James ; Wax, Mark. / Effect of anemia on the fasciocutaneous flap survival in a rat model. In: Laryngoscope. 2001 ; Vol. 111, No. 4 I. pp. 572-575.
@article{966121d658cb49aeabc536192951cf34,
title = "Effect of anemia on the fasciocutaneous flap survival in a rat model",
abstract = "Objective/Hypothesis: Ablative surgery for head and neck cancer that creates large composite defects often results in a significant decrease in the hematocrit level. These defects are best reconstructed with a microvascular free tissue transfer. Effect of the decreased hematocrit on microvascular flap survival is controversial. The purpose of this study was to assess the effect of isovolemic anemia on flap survival in a rat model. Study Design: Prospective analysis. Methods: Ninety rats were used (30 control and 60 experimental animals). Experimental animals were rendered anemic by blood draw and volume resuscitated with either a colloid (30 animals) or crystalloid (30 animals) solution. In all animals a ventral fasciocutaneous flap was raised. A vascular clamp was applied to the arteriovenous pedicle, and different ischemic times were allowed to elapse before clamp removal. Flap survival was assessed at 5 days. Probit analysis was performed for the three animal groups. Results: A significantly increased probability of the flap survival was found in the anemic animals compared with the control group (P ≤.05). No difference was found between the colloid and crystalloid resuscitation groups. Conclusions: A decreased hematocrit level increases fasciocutaneous flap tolerance to ischemia and significantly increases the primary ischemic time in the ventral flap clamp model in rats. Fluid replacement with either crystalloid or colloid produces identical results.",
keywords = "Anemia, Animal model, Free tissue transfer, Hematocrit",
author = "S. Desyatnikova and C. Winslow and James Cohen and Mark Wax",
year = "2001",
language = "English (US)",
volume = "111",
pages = "572--575",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "4 I",

}

TY - JOUR

T1 - Effect of anemia on the fasciocutaneous flap survival in a rat model

AU - Desyatnikova, S.

AU - Winslow, C.

AU - Cohen, James

AU - Wax, Mark

PY - 2001

Y1 - 2001

N2 - Objective/Hypothesis: Ablative surgery for head and neck cancer that creates large composite defects often results in a significant decrease in the hematocrit level. These defects are best reconstructed with a microvascular free tissue transfer. Effect of the decreased hematocrit on microvascular flap survival is controversial. The purpose of this study was to assess the effect of isovolemic anemia on flap survival in a rat model. Study Design: Prospective analysis. Methods: Ninety rats were used (30 control and 60 experimental animals). Experimental animals were rendered anemic by blood draw and volume resuscitated with either a colloid (30 animals) or crystalloid (30 animals) solution. In all animals a ventral fasciocutaneous flap was raised. A vascular clamp was applied to the arteriovenous pedicle, and different ischemic times were allowed to elapse before clamp removal. Flap survival was assessed at 5 days. Probit analysis was performed for the three animal groups. Results: A significantly increased probability of the flap survival was found in the anemic animals compared with the control group (P ≤.05). No difference was found between the colloid and crystalloid resuscitation groups. Conclusions: A decreased hematocrit level increases fasciocutaneous flap tolerance to ischemia and significantly increases the primary ischemic time in the ventral flap clamp model in rats. Fluid replacement with either crystalloid or colloid produces identical results.

AB - Objective/Hypothesis: Ablative surgery for head and neck cancer that creates large composite defects often results in a significant decrease in the hematocrit level. These defects are best reconstructed with a microvascular free tissue transfer. Effect of the decreased hematocrit on microvascular flap survival is controversial. The purpose of this study was to assess the effect of isovolemic anemia on flap survival in a rat model. Study Design: Prospective analysis. Methods: Ninety rats were used (30 control and 60 experimental animals). Experimental animals were rendered anemic by blood draw and volume resuscitated with either a colloid (30 animals) or crystalloid (30 animals) solution. In all animals a ventral fasciocutaneous flap was raised. A vascular clamp was applied to the arteriovenous pedicle, and different ischemic times were allowed to elapse before clamp removal. Flap survival was assessed at 5 days. Probit analysis was performed for the three animal groups. Results: A significantly increased probability of the flap survival was found in the anemic animals compared with the control group (P ≤.05). No difference was found between the colloid and crystalloid resuscitation groups. Conclusions: A decreased hematocrit level increases fasciocutaneous flap tolerance to ischemia and significantly increases the primary ischemic time in the ventral flap clamp model in rats. Fluid replacement with either crystalloid or colloid produces identical results.

KW - Anemia

KW - Animal model

KW - Free tissue transfer

KW - Hematocrit

UR - http://www.scopus.com/inward/record.url?scp=0035070692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035070692&partnerID=8YFLogxK

M3 - Article

C2 - 11359122

AN - SCOPUS:0035070692

VL - 111

SP - 572

EP - 575

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 4 I

ER -