Effect of an anti-insulin-like growth factor I receptor antibody on insulin-like growth factor II stimulation of DNA synthesis in human fibroblasts

C. A. Conover, P. Misra, R. L. Hintz, R. G. Rosenfeld

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

To investigate the role of insulin-like growth factor II in the control of DNA synthesis in human fibroblasts, dose-response curves for insulin-like growth factor I and II stimulation of [3H]thymidine incorporation were compared in the absence and presence of alphaIR-3, a highly specific monoclonal antibody directed against the type I insulin-like growth factor receptor. Specific binding of [125I]insulin-like growth factor I to human fibroblast monolayer cultures was inhibited 60-70% in the presence of alphaIR-3. alphaIR-3 had no effect on [125I]insulin-like growth factor II binding to human fibroblasts. However, alphaIR-3 inhibited both insulin-like growth factor I and II stimulated [3H]thymidine incorporation. These data indicate that the type II insulin-like growth factor receptor does not function as a transducer of insulin-like growth factor II's mitogenic effect in human fibroblasts.

Original languageEnglish (US)
Pages (from-to)501-508
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number2
DOIs
StatePublished - Sep 16 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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