Effect of an anti-insulin-like growth factor I receptor antibody on insulin-like growth factor II stimulation of DNA synthesis in human fibroblasts

C. A. Conover, P. Misra, R. L. Hintz, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

To investigate the role of insulin-like growth factor II in the control of DNA synthesis in human fibroblasts, dose-response curves for insulin-like growth factor I and II stimulation of [3H]thymidine incorporation were compared in the absence and presence of alphaIR-3, a highly specific monoclonal antibody directed against the type I insulin-like growth factor receptor. Specific binding of [125I]insulin-like growth factor I to human fibroblast monolayer cultures was inhibited 60-70% in the presence of alphaIR-3. alphaIR-3 had no effect on [125I]insulin-like growth factor II binding to human fibroblasts. However, alphaIR-3 inhibited both insulin-like growth factor I and II stimulated [3H]thymidine incorporation. These data indicate that the type II insulin-like growth factor receptor does not function as a transducer of insulin-like growth factor II's mitogenic effect in human fibroblasts.

Original languageEnglish (US)
Pages (from-to)501-508
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number2
DOIs
StatePublished - Sep 16 1986
Externally publishedYes

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IGF Type 1 Receptor
Insulin-Like Growth Factor II
Fibroblasts
Insulin-Like Growth Factor I
Antibodies
DNA
Thymidine
IGF Type 2 Receptor
Transducers
Cell culture
Monolayers
Monoclonal Antibodies

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Biochemistry

Cite this

Effect of an anti-insulin-like growth factor I receptor antibody on insulin-like growth factor II stimulation of DNA synthesis in human fibroblasts. / Conover, C. A.; Misra, P.; Hintz, R. L.; Rosenfeld, Ronald (Ron).

In: Biochemical and Biophysical Research Communications, Vol. 139, No. 2, 16.09.1986, p. 501-508.

Research output: Contribution to journalArticle

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