Effect of a tyrosine kinase inhibitor, genistein, on the actions of prolactin in cultured mouse mammary tissues

Guang Fan, James A. Rillema

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Genistein, an inhibitor of tyrosine kinase, was used to determine the possible role of tyrosine kinase in the prolactin (PRL) stimulation of milk product formation and ornithine decarboxylase (ODC) activation in cultured mouse mammary gland tissue. Genistein (10-200 μM) inhibited in a dose-response fashion the PRL stimulation of casein, lipid and lactose synthesis as well as ODC activation. Genistein, however, did not inhibit the phospholipase C, phorbol myristate acetate or cAMP effects on ODC activation. These results suggest the possible involvement of tyrosine kinase in the mechanism by which PRL expresses its effects in mammary gland tissues.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume83
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

Keywords

  • Genistein
  • Mammary gland tissue
  • Prolactin
  • Tyrosine kinase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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