Effect of a single bolus of intracoronary basic fibroblast growth factor on perfusion in an ischemic porcine model

Gerard P. Devlin, Stephen Fort, Eric Yu, Robert J. Cusimano, Kevin Wei, Harry Rakowski, Jack Butany, Janice A. Richards, Michael J. Sole, Leonard Schwartz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Basic fibroblast growth factor (bFGF) has been shown to induce angiogenesis in various animal models, but the methods of administration used experimentally are not clinically feasible. The objective of this study was to determine whether a single intracoronary bolus injection of bFGF would improve coronary perfusion in a porcine ischemic model that mimics clinical chronic ischemia. A copper coil studded with gold was delivered into the proximal right coronary artery of juvenile Yorkshire pigs and deployed by interventional techniques. After a four-week interval for stenosis maturation, bFGF (100 μg) was administered by bolus injection into the left coronary artery in five animals, and vehicle alone was administered in four animals. Angiogenesis and change in right coronary perfusion area were assessed two weeks later by angiography, myocardial contrast echocardiography and immunohistochemistry. The right coronary perfusion area increased significantly after treatment in all but one of the animals that received bFGF but not in any of the controls. Intimal hyperplasia was not induced by bFGF. Capillary density determined histochemically was not different in the two groups. In conclusion, in a porcine ischemic model, bFGF administered by a single bolus intracoronary injection into the contralateral artery improved antegrade perfusion into the ischemic territory although without histological evidence of angiogenesis. This preliminary work merits further investigation.

Original languageEnglish (US)
Pages (from-to)676-682
Number of pages7
JournalCanadian Journal of Cardiology
Volume15
Issue number6
StatePublished - Jun 1999
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Swine
Perfusion
Injections
Coronary Vessels
Tunica Intima
Gold
Hyperplasia
Echocardiography
Copper
Angiography
Pathologic Constriction
Ischemia
Animal Models
Arteries
Immunohistochemistry

Keywords

  • Angiogenesis
  • Growth substances
  • Ischemia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Devlin, G. P., Fort, S., Yu, E., Cusimano, R. J., Wei, K., Rakowski, H., ... Schwartz, L. (1999). Effect of a single bolus of intracoronary basic fibroblast growth factor on perfusion in an ischemic porcine model. Canadian Journal of Cardiology, 15(6), 676-682.

Effect of a single bolus of intracoronary basic fibroblast growth factor on perfusion in an ischemic porcine model. / Devlin, Gerard P.; Fort, Stephen; Yu, Eric; Cusimano, Robert J.; Wei, Kevin; Rakowski, Harry; Butany, Jack; Richards, Janice A.; Sole, Michael J.; Schwartz, Leonard.

In: Canadian Journal of Cardiology, Vol. 15, No. 6, 06.1999, p. 676-682.

Research output: Contribution to journalArticle

Devlin, GP, Fort, S, Yu, E, Cusimano, RJ, Wei, K, Rakowski, H, Butany, J, Richards, JA, Sole, MJ & Schwartz, L 1999, 'Effect of a single bolus of intracoronary basic fibroblast growth factor on perfusion in an ischemic porcine model', Canadian Journal of Cardiology, vol. 15, no. 6, pp. 676-682.
Devlin, Gerard P. ; Fort, Stephen ; Yu, Eric ; Cusimano, Robert J. ; Wei, Kevin ; Rakowski, Harry ; Butany, Jack ; Richards, Janice A. ; Sole, Michael J. ; Schwartz, Leonard. / Effect of a single bolus of intracoronary basic fibroblast growth factor on perfusion in an ischemic porcine model. In: Canadian Journal of Cardiology. 1999 ; Vol. 15, No. 6. pp. 676-682.
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