Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs

P. J. Syapin, B. L. Jones, Deborah (Deb) Finn, D. L. Davies, R. L. Alkana

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The mechanism by which 12 atm abs of a helium-oxygen gas mixture (heliox) antagonizes behavioral effects of ethanol is unknown. Although the threshold for pressure-reversal of general anesthesia and expression of the high pressure neurologic syndrome (HPNS) is well above 12 atm abs in mice, the ethanol antagonism by 12 atm abs heliox could result from similar underlying excitatory effects. To investigate this possibility, the behavior of water-injected control mice and the latency to convulsions in drug-injected mice were determined in 1 atm abs air and 12 atm abs heliox. Four convulsant drugs were tested: picrotoxin (2 mg/kg), dl-allylglycine (300 mg/kg), isoniazid (300 mg/kg), and l-methionine-dl-sulfoximine (170 mg/kg). Responses were videotaped to observe behavior and to measure latency to the onset of myoclonus and clonus. Results indicated no observable excitatory effects of 12 atm abs in control mice. The latency to myoclonus was significantly reduced by pressure in allylglycine-treated mice but not in mice treated with the other convulsants. Latency to clonus was not significantly altered by pressure, relative to latency at 1 atm abs heliox, for any drug tested. In conclusion, the present findings indicate that exposure to 12 atm abs heliox is not proconvulsant and, thus, the findings do not support the hypothesis that 12 atm abs heliox antagonizes ethanol indirectly via an increase in central nervous system excitability.

Original languageEnglish (US)
Pages (from-to)34-41
Number of pages8
JournalUndersea and Hyperbaric Medicine
Volume23
Issue number1
StatePublished - Mar 1996

Fingerprint

Convulsants
Helium
helium
Atmosphere
ethanol
drug
Oxygen
oxygen
drugs
atmosphere
Allylglycine
mice
Pharmaceutical Preparations
Pressure
Myoclonus
Ethanol
antagonism
nervous system
Methionine Sulfoximine
isoniazid

Keywords

  • Alcohol antagonist
  • Clonic convulsions
  • Convulsant drug effects
  • Drug lethality
  • Hyperbaric exposure

ASJC Scopus subject areas

  • Aquatic Science
  • Medicine (miscellaneous)

Cite this

Syapin, P. J., Jones, B. L., Finn, D. D., Davies, D. L., & Alkana, R. L. (1996). Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs. Undersea and Hyperbaric Medicine, 23(1), 34-41.

Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs. / Syapin, P. J.; Jones, B. L.; Finn, Deborah (Deb); Davies, D. L.; Alkana, R. L.

In: Undersea and Hyperbaric Medicine, Vol. 23, No. 1, 03.1996, p. 34-41.

Research output: Contribution to journalArticle

Syapin, PJ, Jones, BL, Finn, DD, Davies, DL & Alkana, RL 1996, 'Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs', Undersea and Hyperbaric Medicine, vol. 23, no. 1, pp. 34-41.
Syapin, P. J. ; Jones, B. L. ; Finn, Deborah (Deb) ; Davies, D. L. ; Alkana, R. L. / Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs. In: Undersea and Hyperbaric Medicine. 1996 ; Vol. 23, No. 1. pp. 34-41.
@article{4dda4a9da73147b89319539d4c8141d1,
title = "Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs",
abstract = "The mechanism by which 12 atm abs of a helium-oxygen gas mixture (heliox) antagonizes behavioral effects of ethanol is unknown. Although the threshold for pressure-reversal of general anesthesia and expression of the high pressure neurologic syndrome (HPNS) is well above 12 atm abs in mice, the ethanol antagonism by 12 atm abs heliox could result from similar underlying excitatory effects. To investigate this possibility, the behavior of water-injected control mice and the latency to convulsions in drug-injected mice were determined in 1 atm abs air and 12 atm abs heliox. Four convulsant drugs were tested: picrotoxin (2 mg/kg), dl-allylglycine (300 mg/kg), isoniazid (300 mg/kg), and l-methionine-dl-sulfoximine (170 mg/kg). Responses were videotaped to observe behavior and to measure latency to the onset of myoclonus and clonus. Results indicated no observable excitatory effects of 12 atm abs in control mice. The latency to myoclonus was significantly reduced by pressure in allylglycine-treated mice but not in mice treated with the other convulsants. Latency to clonus was not significantly altered by pressure, relative to latency at 1 atm abs heliox, for any drug tested. In conclusion, the present findings indicate that exposure to 12 atm abs heliox is not proconvulsant and, thus, the findings do not support the hypothesis that 12 atm abs heliox antagonizes ethanol indirectly via an increase in central nervous system excitability.",
keywords = "Alcohol antagonist, Clonic convulsions, Convulsant drug effects, Drug lethality, Hyperbaric exposure",
author = "Syapin, {P. J.} and Jones, {B. L.} and Finn, {Deborah (Deb)} and Davies, {D. L.} and Alkana, {R. L.}",
year = "1996",
month = "3",
language = "English (US)",
volume = "23",
pages = "34--41",
journal = "Undersea and Hyperbaric Medicine",
issn = "1066-2936",
publisher = "Undersea and Hyperbaric Medical Society",
number = "1",

}

TY - JOUR

T1 - Effect of 12 atmospheres helium-oxygen on the response of mice to convulsant drugs

AU - Syapin, P. J.

AU - Jones, B. L.

AU - Finn, Deborah (Deb)

AU - Davies, D. L.

AU - Alkana, R. L.

PY - 1996/3

Y1 - 1996/3

N2 - The mechanism by which 12 atm abs of a helium-oxygen gas mixture (heliox) antagonizes behavioral effects of ethanol is unknown. Although the threshold for pressure-reversal of general anesthesia and expression of the high pressure neurologic syndrome (HPNS) is well above 12 atm abs in mice, the ethanol antagonism by 12 atm abs heliox could result from similar underlying excitatory effects. To investigate this possibility, the behavior of water-injected control mice and the latency to convulsions in drug-injected mice were determined in 1 atm abs air and 12 atm abs heliox. Four convulsant drugs were tested: picrotoxin (2 mg/kg), dl-allylglycine (300 mg/kg), isoniazid (300 mg/kg), and l-methionine-dl-sulfoximine (170 mg/kg). Responses were videotaped to observe behavior and to measure latency to the onset of myoclonus and clonus. Results indicated no observable excitatory effects of 12 atm abs in control mice. The latency to myoclonus was significantly reduced by pressure in allylglycine-treated mice but not in mice treated with the other convulsants. Latency to clonus was not significantly altered by pressure, relative to latency at 1 atm abs heliox, for any drug tested. In conclusion, the present findings indicate that exposure to 12 atm abs heliox is not proconvulsant and, thus, the findings do not support the hypothesis that 12 atm abs heliox antagonizes ethanol indirectly via an increase in central nervous system excitability.

AB - The mechanism by which 12 atm abs of a helium-oxygen gas mixture (heliox) antagonizes behavioral effects of ethanol is unknown. Although the threshold for pressure-reversal of general anesthesia and expression of the high pressure neurologic syndrome (HPNS) is well above 12 atm abs in mice, the ethanol antagonism by 12 atm abs heliox could result from similar underlying excitatory effects. To investigate this possibility, the behavior of water-injected control mice and the latency to convulsions in drug-injected mice were determined in 1 atm abs air and 12 atm abs heliox. Four convulsant drugs were tested: picrotoxin (2 mg/kg), dl-allylglycine (300 mg/kg), isoniazid (300 mg/kg), and l-methionine-dl-sulfoximine (170 mg/kg). Responses were videotaped to observe behavior and to measure latency to the onset of myoclonus and clonus. Results indicated no observable excitatory effects of 12 atm abs in control mice. The latency to myoclonus was significantly reduced by pressure in allylglycine-treated mice but not in mice treated with the other convulsants. Latency to clonus was not significantly altered by pressure, relative to latency at 1 atm abs heliox, for any drug tested. In conclusion, the present findings indicate that exposure to 12 atm abs heliox is not proconvulsant and, thus, the findings do not support the hypothesis that 12 atm abs heliox antagonizes ethanol indirectly via an increase in central nervous system excitability.

KW - Alcohol antagonist

KW - Clonic convulsions

KW - Convulsant drug effects

KW - Drug lethality

KW - Hyperbaric exposure

UR - http://www.scopus.com/inward/record.url?scp=0030097740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030097740&partnerID=8YFLogxK

M3 - Article

C2 - 8653064

AN - SCOPUS:0030097740

VL - 23

SP - 34

EP - 41

JO - Undersea and Hyperbaric Medicine

JF - Undersea and Hyperbaric Medicine

SN - 1066-2936

IS - 1

ER -