EEDQ reduces the striatal neurotensin mRNA response to haloperidol

Monique R. Adams, Dorcas J. Dobie, Kalpana M. Merchant, Alan Unis, Daniel Dorsa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Haloperidol is believed to induce neurotensin/neuromedin N (NT/N) gene expression in the dorsolateral striatum (DLST) of the rat brain via dopamine D2 receptor blockade, but is also known to interact with other receptors as well. To further characterize haloperidol's effects, rats were treated with the irreversible monoaminergic receptor antagonist N-ethoxycarbonyl-2-ethoxy-1,2 hydroxyquinolone (EEDQ). In situ hybridization was performed for NT/N mRNA. D2-like and sigma receptor autoradiography was performed using 125I-sulpride and 3H-1,3-di-o-tolylguanidine (DTG), respectively. Despite antagonism of D2 receptors, pretreatment with EEDQ failed to significantly reduce the NT/N mRNA response when given 3 days prior to the haloperidol challenge. These data suggest that the acute effects of haloperidol on NT/N mRNA expression in large part result from D2 receptor antagonism. Nonetheless, a contribution of other receptors can not be excluded.

Original languageEnglish (US)
Pages (from-to)527-535
Number of pages9
JournalPeptides
Volume18
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

neuromedin N
Corpus Striatum
Neurotensin
Haloperidol
Messenger RNA
Rats
sigma Receptors
Dopamine D2 Receptors
Autoradiography
Gene expression
In Situ Hybridization
Brain
Gene Expression
EEDQ

Keywords

  • Antipsychotic
  • Dopamine
  • Dorsolateral striatum
  • Drugs Haloperidol
  • EEDQ
  • Neurotensin/neuromedin N

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

EEDQ reduces the striatal neurotensin mRNA response to haloperidol. / Adams, Monique R.; Dobie, Dorcas J.; Merchant, Kalpana M.; Unis, Alan; Dorsa, Daniel.

In: Peptides, Vol. 18, No. 4, 1997, p. 527-535.

Research output: Contribution to journalArticle

Adams, Monique R. ; Dobie, Dorcas J. ; Merchant, Kalpana M. ; Unis, Alan ; Dorsa, Daniel. / EEDQ reduces the striatal neurotensin mRNA response to haloperidol. In: Peptides. 1997 ; Vol. 18, No. 4. pp. 527-535.
@article{112559cf29474c79b78f1e38b3c90ab8,
title = "EEDQ reduces the striatal neurotensin mRNA response to haloperidol",
abstract = "Haloperidol is believed to induce neurotensin/neuromedin N (NT/N) gene expression in the dorsolateral striatum (DLST) of the rat brain via dopamine D2 receptor blockade, but is also known to interact with other receptors as well. To further characterize haloperidol's effects, rats were treated with the irreversible monoaminergic receptor antagonist N-ethoxycarbonyl-2-ethoxy-1,2 hydroxyquinolone (EEDQ). In situ hybridization was performed for NT/N mRNA. D2-like and sigma receptor autoradiography was performed using 125I-sulpride and 3H-1,3-di-o-tolylguanidine (DTG), respectively. Despite antagonism of D2 receptors, pretreatment with EEDQ failed to significantly reduce the NT/N mRNA response when given 3 days prior to the haloperidol challenge. These data suggest that the acute effects of haloperidol on NT/N mRNA expression in large part result from D2 receptor antagonism. Nonetheless, a contribution of other receptors can not be excluded.",
keywords = "Antipsychotic, Dopamine, Dorsolateral striatum, Drugs Haloperidol, EEDQ, Neurotensin/neuromedin N",
author = "Adams, {Monique R.} and Dobie, {Dorcas J.} and Merchant, {Kalpana M.} and Alan Unis and Daniel Dorsa",
year = "1997",
doi = "10.1016/S0196-9781(97)87957-0",
language = "English (US)",
volume = "18",
pages = "527--535",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - EEDQ reduces the striatal neurotensin mRNA response to haloperidol

AU - Adams, Monique R.

AU - Dobie, Dorcas J.

AU - Merchant, Kalpana M.

AU - Unis, Alan

AU - Dorsa, Daniel

PY - 1997

Y1 - 1997

N2 - Haloperidol is believed to induce neurotensin/neuromedin N (NT/N) gene expression in the dorsolateral striatum (DLST) of the rat brain via dopamine D2 receptor blockade, but is also known to interact with other receptors as well. To further characterize haloperidol's effects, rats were treated with the irreversible monoaminergic receptor antagonist N-ethoxycarbonyl-2-ethoxy-1,2 hydroxyquinolone (EEDQ). In situ hybridization was performed for NT/N mRNA. D2-like and sigma receptor autoradiography was performed using 125I-sulpride and 3H-1,3-di-o-tolylguanidine (DTG), respectively. Despite antagonism of D2 receptors, pretreatment with EEDQ failed to significantly reduce the NT/N mRNA response when given 3 days prior to the haloperidol challenge. These data suggest that the acute effects of haloperidol on NT/N mRNA expression in large part result from D2 receptor antagonism. Nonetheless, a contribution of other receptors can not be excluded.

AB - Haloperidol is believed to induce neurotensin/neuromedin N (NT/N) gene expression in the dorsolateral striatum (DLST) of the rat brain via dopamine D2 receptor blockade, but is also known to interact with other receptors as well. To further characterize haloperidol's effects, rats were treated with the irreversible monoaminergic receptor antagonist N-ethoxycarbonyl-2-ethoxy-1,2 hydroxyquinolone (EEDQ). In situ hybridization was performed for NT/N mRNA. D2-like and sigma receptor autoradiography was performed using 125I-sulpride and 3H-1,3-di-o-tolylguanidine (DTG), respectively. Despite antagonism of D2 receptors, pretreatment with EEDQ failed to significantly reduce the NT/N mRNA response when given 3 days prior to the haloperidol challenge. These data suggest that the acute effects of haloperidol on NT/N mRNA expression in large part result from D2 receptor antagonism. Nonetheless, a contribution of other receptors can not be excluded.

KW - Antipsychotic

KW - Dopamine

KW - Dorsolateral striatum

KW - Drugs Haloperidol

KW - EEDQ

KW - Neurotensin/neuromedin N

UR - http://www.scopus.com/inward/record.url?scp=0030973303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030973303&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(97)87957-0

DO - 10.1016/S0196-9781(97)87957-0

M3 - Article

C2 - 9210171

AN - SCOPUS:0030973303

VL - 18

SP - 527

EP - 535

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 4

ER -