Ectopic expression of SOD and APX genes in Arabidopsis alters metabolic pools and genes related to secondary cell wall cellulose biosynthesis and improve salt tolerance

Amrina Shafi, Tejpal Gill, Insha Zahoor, Paramvir Singh Ahuja, Yelam Sreenivasulu, Sanjay Kumar, Anil Kumar Singh

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Hydrogen peroxide (H 2 O 2 ) is known to accumulate in plants during abiotic stress conditions and also acts as a signalling molecule. In this study, Arabidopsis thaliana transgenics overexpressing cytosolic CuZn-superoxide dismutase (PaSOD) from poly-extremophile high-altitude Himalayan plant Potentilla atrosanguinea, cytosolic ascorbate peroxidase (RaAPX) from Rheum australe and dual transgenics overexpressing both the genes were developed and analyzed under salt stress. In comparison to wild-type (WT) or single transgenics, the performance of dual transgenics under salt stress was better with higher biomass accumulation and cellulose content. We identified genes involved in cell wall biosynthesis, including nine cellulose synthases (CesA), seven cellulose synthase-like proteins together with other wall-related genes. RNA-seq analysis and qPCR revealed differential regulation of genes (CesA 4, 7 and 8) and transcription factors (MYB46 and 83) involved in secondary cell wall cellulose biosynthesis, amongst which most of the cellulose biosynthesis gene showed upregulation in single (PaSOD line) and dual transgenics at 100 mM salt stress. A positive correlation between cellulose content and H 2 O 2 accumulation was observed in these transgenic lines. Further, cellulose content was 1.6–2 folds significantly higher in PaSOD and dual transgenic lines, 1.4 fold higher in RaAPX lines as compared to WT plants under stress conditions. Additionally, transgenics overexpressing PaSOD and RaAPX also displayed higher amounts of phenolics as compared to WT. The novelty of present study is that H 2 O 2 apart from its role in signalling, it also provides mechanical strength to plants and aid in plant biomass production during salt stress by transcriptional activation of cellulose biosynthesis pathway. This modulation of the cellulose biosynthetic machinery in plants has the potential to provide insight into plant growth, morphogenesis and to create plants with enhanced cellulose content for biofuel use.

Original languageEnglish (US)
Pages (from-to)1985-2002
Number of pages18
JournalMolecular Biology Reports
Volume46
Issue number2
DOIs
StatePublished - Apr 1 2019
Externally publishedYes

Keywords

  • APX
  • Cell wall biosynthesis
  • CuZn-SOD
  • Flavonoids
  • H O signalling
  • Salinity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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