Ectoparasite burden, clinical disease, and immune responses throughout fur mite (Myocoptes musculinus) infestation in C57BL/6 and Rag1-/- mice

Cassandra Moats, Victoria K. Baxter, Nathan M. Pate, Julie Watson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Immunocompetent weanling mice infested with Myocoptes musculinus harbor high mite loads, yet burdens decrease with age. The development of immunity to the parasite may explain this observation. In this study, we followed M. musculinus burdens in Rag1-/- mice and immunocompetent C57BL/6 controls from 4 to 36 wk of age and compared the clinical signs and body weights of noninfested and infested mice of both strains over time. In addition, histopathology of skin lesions and expression of cytokines and transcription factors associated with Th1- And Th2- Type immune responses were assessed. Myocoptes burdens decreased and remained low in B6 mice over time, whereas Rag1-/- mice showed an initial decrease in burdens after 4 wk of age followed by an increase from 24 to 36 wk. In addition, Rag1-/- mice had higher burdens than B6 mice over time. Both strains of infested mice exhibited clinical signs of fur mite infestation-including alopecia, poor weight gain, mite- Associated debris, and pruritus- And clinical signs positively correlated with the severity of the Myocoptes burden. Histopathology of skin from both strains of infested mice showed decreased lesion severity with age, likely a result of declining mite populations. Finally, compared with noninfested controls, infested B6 mice had increased expression of markers associated with the Th2- Type immune response, which increased in magnitude with increasing age and duration of infestation. These results suggest that development of adaptive immunity plays a role in control of fur mite populations and that heavier infestations may result in more severe clinical signs and skin lesions.

Original languageEnglish (US)
Pages (from-to)197-207
Number of pages11
JournalComparative Medicine
Volume66
Issue number3
StatePublished - Jun 1 2016
Externally publishedYes

Fingerprint

Mites
Immune System Diseases
ectoparasites
fur
mites
Skin
immune response
mice
Ports and harbors
Myocoptes
Debris
Transcription Factors
Cytokines
skin lesions
histopathology
Tick Control
Mite Infestations
Myocoptes musculinus
mite infestations
Alopecia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Ectoparasite burden, clinical disease, and immune responses throughout fur mite (Myocoptes musculinus) infestation in C57BL/6 and Rag1-/- mice. / Moats, Cassandra; Baxter, Victoria K.; Pate, Nathan M.; Watson, Julie.

In: Comparative Medicine, Vol. 66, No. 3, 01.06.2016, p. 197-207.

Research output: Contribution to journalArticle

@article{f5a3d1e3652e436080572eadfe9ede49,
title = "Ectoparasite burden, clinical disease, and immune responses throughout fur mite (Myocoptes musculinus) infestation in C57BL/6 and Rag1-/- mice",
abstract = "Immunocompetent weanling mice infested with Myocoptes musculinus harbor high mite loads, yet burdens decrease with age. The development of immunity to the parasite may explain this observation. In this study, we followed M. musculinus burdens in Rag1-/- mice and immunocompetent C57BL/6 controls from 4 to 36 wk of age and compared the clinical signs and body weights of noninfested and infested mice of both strains over time. In addition, histopathology of skin lesions and expression of cytokines and transcription factors associated with Th1- And Th2- Type immune responses were assessed. Myocoptes burdens decreased and remained low in B6 mice over time, whereas Rag1-/- mice showed an initial decrease in burdens after 4 wk of age followed by an increase from 24 to 36 wk. In addition, Rag1-/- mice had higher burdens than B6 mice over time. Both strains of infested mice exhibited clinical signs of fur mite infestation-including alopecia, poor weight gain, mite- Associated debris, and pruritus- And clinical signs positively correlated with the severity of the Myocoptes burden. Histopathology of skin from both strains of infested mice showed decreased lesion severity with age, likely a result of declining mite populations. Finally, compared with noninfested controls, infested B6 mice had increased expression of markers associated with the Th2- Type immune response, which increased in magnitude with increasing age and duration of infestation. These results suggest that development of adaptive immunity plays a role in control of fur mite populations and that heavier infestations may result in more severe clinical signs and skin lesions.",
author = "Cassandra Moats and Baxter, {Victoria K.} and Pate, {Nathan M.} and Julie Watson",
year = "2016",
month = "6",
day = "1",
language = "English (US)",
volume = "66",
pages = "197--207",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "3",

}

TY - JOUR

T1 - Ectoparasite burden, clinical disease, and immune responses throughout fur mite (Myocoptes musculinus) infestation in C57BL/6 and Rag1-/- mice

AU - Moats, Cassandra

AU - Baxter, Victoria K.

AU - Pate, Nathan M.

AU - Watson, Julie

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Immunocompetent weanling mice infested with Myocoptes musculinus harbor high mite loads, yet burdens decrease with age. The development of immunity to the parasite may explain this observation. In this study, we followed M. musculinus burdens in Rag1-/- mice and immunocompetent C57BL/6 controls from 4 to 36 wk of age and compared the clinical signs and body weights of noninfested and infested mice of both strains over time. In addition, histopathology of skin lesions and expression of cytokines and transcription factors associated with Th1- And Th2- Type immune responses were assessed. Myocoptes burdens decreased and remained low in B6 mice over time, whereas Rag1-/- mice showed an initial decrease in burdens after 4 wk of age followed by an increase from 24 to 36 wk. In addition, Rag1-/- mice had higher burdens than B6 mice over time. Both strains of infested mice exhibited clinical signs of fur mite infestation-including alopecia, poor weight gain, mite- Associated debris, and pruritus- And clinical signs positively correlated with the severity of the Myocoptes burden. Histopathology of skin from both strains of infested mice showed decreased lesion severity with age, likely a result of declining mite populations. Finally, compared with noninfested controls, infested B6 mice had increased expression of markers associated with the Th2- Type immune response, which increased in magnitude with increasing age and duration of infestation. These results suggest that development of adaptive immunity plays a role in control of fur mite populations and that heavier infestations may result in more severe clinical signs and skin lesions.

AB - Immunocompetent weanling mice infested with Myocoptes musculinus harbor high mite loads, yet burdens decrease with age. The development of immunity to the parasite may explain this observation. In this study, we followed M. musculinus burdens in Rag1-/- mice and immunocompetent C57BL/6 controls from 4 to 36 wk of age and compared the clinical signs and body weights of noninfested and infested mice of both strains over time. In addition, histopathology of skin lesions and expression of cytokines and transcription factors associated with Th1- And Th2- Type immune responses were assessed. Myocoptes burdens decreased and remained low in B6 mice over time, whereas Rag1-/- mice showed an initial decrease in burdens after 4 wk of age followed by an increase from 24 to 36 wk. In addition, Rag1-/- mice had higher burdens than B6 mice over time. Both strains of infested mice exhibited clinical signs of fur mite infestation-including alopecia, poor weight gain, mite- Associated debris, and pruritus- And clinical signs positively correlated with the severity of the Myocoptes burden. Histopathology of skin from both strains of infested mice showed decreased lesion severity with age, likely a result of declining mite populations. Finally, compared with noninfested controls, infested B6 mice had increased expression of markers associated with the Th2- Type immune response, which increased in magnitude with increasing age and duration of infestation. These results suggest that development of adaptive immunity plays a role in control of fur mite populations and that heavier infestations may result in more severe clinical signs and skin lesions.

UR - http://www.scopus.com/inward/record.url?scp=84978977035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978977035&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 197

EP - 207

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 3

ER -