Echocardiographic evaluation of the effects of stem cell therapy on perfusion and function in ischemic cardiomyopathy

Yoichi Inaba, Brian P. Davidson, Sajeevani Kim, Ya Ni Liu, William Packwood, J. Todd Belcik, Aris Xie, Jonathan Lindner

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Background Small animal models of ischemic left ventricular (LV) dysfunction are important for the preclinical optimization of stem cell therapy. The aim of this study was to test the hypothesis that temporal changes in LV function and regional perfusion after cell therapy can be assessed in mice using echocardiographic imaging. Methods Wild-type mice (n = 25) were studied 7 and 28 days after permanent ligation of the left anterior descending coronary artery. Animals were randomized to receive closed-chest ultrasound-guided intramyocardial delivery of saline (n = 13) or 5 × 105 multipotential adult progenitor cells (MAPCs; n = 12) on day 7. LV end-diastolic and end-systolic volumes, LV ejection fraction, and stroke volume were measured using high-frequency echocardiography. Multiplanar assessments of perfusion and defect area size were made using myocardial contrast echocardiography. Results Between days 7 and 28, MAPC-treated animals had 40% to 50% reductions in defect size (P

    Original languageEnglish (US)
    Pages (from-to)192-199
    Number of pages8
    JournalJournal of the American Society of Echocardiography
    Volume27
    Issue number2
    DOIs
    StatePublished - Feb 2014

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    Cell- and Tissue-Based Therapy
    Cardiomyopathies
    Stroke Volume
    Echocardiography
    Stem Cells
    Perfusion
    Left Ventricular Dysfunction
    Left Ventricular Function
    Ligation
    Coronary Vessels
    Thorax
    Animal Models

    Keywords

    • Contrast echocardiography
    • Echocardiography
    • Microbubbles
    • Stem cells

    ASJC Scopus subject areas

    • Radiology Nuclear Medicine and imaging
    • Cardiology and Cardiovascular Medicine

    Cite this

    Echocardiographic evaluation of the effects of stem cell therapy on perfusion and function in ischemic cardiomyopathy. / Inaba, Yoichi; Davidson, Brian P.; Kim, Sajeevani; Liu, Ya Ni; Packwood, William; Belcik, J. Todd; Xie, Aris; Lindner, Jonathan.

    In: Journal of the American Society of Echocardiography, Vol. 27, No. 2, 02.2014, p. 192-199.

    Research output: Contribution to journalArticle

    Inaba, Yoichi ; Davidson, Brian P. ; Kim, Sajeevani ; Liu, Ya Ni ; Packwood, William ; Belcik, J. Todd ; Xie, Aris ; Lindner, Jonathan. / Echocardiographic evaluation of the effects of stem cell therapy on perfusion and function in ischemic cardiomyopathy. In: Journal of the American Society of Echocardiography. 2014 ; Vol. 27, No. 2. pp. 192-199.
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    abstract = "Background Small animal models of ischemic left ventricular (LV) dysfunction are important for the preclinical optimization of stem cell therapy. The aim of this study was to test the hypothesis that temporal changes in LV function and regional perfusion after cell therapy can be assessed in mice using echocardiographic imaging. Methods Wild-type mice (n = 25) were studied 7 and 28 days after permanent ligation of the left anterior descending coronary artery. Animals were randomized to receive closed-chest ultrasound-guided intramyocardial delivery of saline (n = 13) or 5 × 105 multipotential adult progenitor cells (MAPCs; n = 12) on day 7. LV end-diastolic and end-systolic volumes, LV ejection fraction, and stroke volume were measured using high-frequency echocardiography. Multiplanar assessments of perfusion and defect area size were made using myocardial contrast echocardiography. Results Between days 7 and 28, MAPC-treated animals had 40{\%} to 50{\%} reductions in defect size (P",
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    AU - Inaba, Yoichi

    AU - Davidson, Brian P.

    AU - Kim, Sajeevani

    AU - Liu, Ya Ni

    AU - Packwood, William

    AU - Belcik, J. Todd

    AU - Xie, Aris

    AU - Lindner, Jonathan

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    N2 - Background Small animal models of ischemic left ventricular (LV) dysfunction are important for the preclinical optimization of stem cell therapy. The aim of this study was to test the hypothesis that temporal changes in LV function and regional perfusion after cell therapy can be assessed in mice using echocardiographic imaging. Methods Wild-type mice (n = 25) were studied 7 and 28 days after permanent ligation of the left anterior descending coronary artery. Animals were randomized to receive closed-chest ultrasound-guided intramyocardial delivery of saline (n = 13) or 5 × 105 multipotential adult progenitor cells (MAPCs; n = 12) on day 7. LV end-diastolic and end-systolic volumes, LV ejection fraction, and stroke volume were measured using high-frequency echocardiography. Multiplanar assessments of perfusion and defect area size were made using myocardial contrast echocardiography. Results Between days 7 and 28, MAPC-treated animals had 40% to 50% reductions in defect size (P

    AB - Background Small animal models of ischemic left ventricular (LV) dysfunction are important for the preclinical optimization of stem cell therapy. The aim of this study was to test the hypothesis that temporal changes in LV function and regional perfusion after cell therapy can be assessed in mice using echocardiographic imaging. Methods Wild-type mice (n = 25) were studied 7 and 28 days after permanent ligation of the left anterior descending coronary artery. Animals were randomized to receive closed-chest ultrasound-guided intramyocardial delivery of saline (n = 13) or 5 × 105 multipotential adult progenitor cells (MAPCs; n = 12) on day 7. LV end-diastolic and end-systolic volumes, LV ejection fraction, and stroke volume were measured using high-frequency echocardiography. Multiplanar assessments of perfusion and defect area size were made using myocardial contrast echocardiography. Results Between days 7 and 28, MAPC-treated animals had 40% to 50% reductions in defect size (P

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    KW - Echocardiography

    KW - Microbubbles

    KW - Stem cells

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