Dysregulation of TGF-β activation contributes to pathogenesis in Marfan syndrome

Enid R. Neptune, Pamela A. Frischmeyer, Dan E. Arking, Loretha Myers, Tracie E. Bunton, Barbara Gayraud, Francesco Ramirez, Lynn Sakai, Harry C. Dietz

Research output: Contribution to journalArticle

976 Citations (Scopus)

Abstract

Marfan syndrome is an autosomal dominant disorder of connective tissue caused by mutations in fibrillin-1 (encoded by FBN1 in humans and Fbn1 in mice), a matrix component of extracellular microfibrils. A distinct subgroup of individuals with Marfan syndrome have distal airspace enlargement, historically described as emphysema, which frequently results in spontaneous lung rupture (pneumothorax; refs. 1-3). To investigate the pathogenesis of genetically imposed emphysema, we analyzed the lung phenotype of mice deficient in fibrillin-1, an accepted model of Marfan syndrome. Lung abnormalities are evident in the immediate postnatal period and manifest as a developmental impairment of distal alveolar septation. Aged mice deficient in fibrillin-1 develop destructive emphysema consistent with the view that early developmental perturbations can predispose to late-onset, seemingly acquired phenotypes. We show that mice deficient in fibrillin-1 have marked dysregulation of transforming growth factor-β (TGF-β) activation and signaling, resulting in apoptosis in the developing lung. Perinatal antagonism of TGF-β attenuates apoptosis and rescues alveolar septation in vivo. These data indicate that matrix sequestration of cytokines is crucial to their regulated activation and signaling and that perturbation of this function can contribute to the pathogenesis of disease.

Original languageEnglish (US)
Pages (from-to)407-411
Number of pages5
JournalNature genetics
Volume33
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Fingerprint

Marfan Syndrome
Transforming Growth Factors
Emphysema
Lung
Apoptosis
Microfibrils
Phenotype
Spontaneous Rupture
Pneumothorax
Connective Tissue
Extracellular Matrix
Cytokines
Mutation
Fibrillin-1

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Neptune, E. R., Frischmeyer, P. A., Arking, D. E., Myers, L., Bunton, T. E., Gayraud, B., ... Dietz, H. C. (2003). Dysregulation of TGF-β activation contributes to pathogenesis in Marfan syndrome. Nature genetics, 33(3), 407-411. https://doi.org/10.1038/ng1116

Dysregulation of TGF-β activation contributes to pathogenesis in Marfan syndrome. / Neptune, Enid R.; Frischmeyer, Pamela A.; Arking, Dan E.; Myers, Loretha; Bunton, Tracie E.; Gayraud, Barbara; Ramirez, Francesco; Sakai, Lynn; Dietz, Harry C.

In: Nature genetics, Vol. 33, No. 3, 01.03.2003, p. 407-411.

Research output: Contribution to journalArticle

Neptune, ER, Frischmeyer, PA, Arking, DE, Myers, L, Bunton, TE, Gayraud, B, Ramirez, F, Sakai, L & Dietz, HC 2003, 'Dysregulation of TGF-β activation contributes to pathogenesis in Marfan syndrome', Nature genetics, vol. 33, no. 3, pp. 407-411. https://doi.org/10.1038/ng1116
Neptune ER, Frischmeyer PA, Arking DE, Myers L, Bunton TE, Gayraud B et al. Dysregulation of TGF-β activation contributes to pathogenesis in Marfan syndrome. Nature genetics. 2003 Mar 1;33(3):407-411. https://doi.org/10.1038/ng1116
Neptune, Enid R. ; Frischmeyer, Pamela A. ; Arking, Dan E. ; Myers, Loretha ; Bunton, Tracie E. ; Gayraud, Barbara ; Ramirez, Francesco ; Sakai, Lynn ; Dietz, Harry C. / Dysregulation of TGF-β activation contributes to pathogenesis in Marfan syndrome. In: Nature genetics. 2003 ; Vol. 33, No. 3. pp. 407-411.
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