Dysbiotic bacteria translocate in progressive SIV infection

Z. Klase, A. Ortiz, C. Deleage, J. C. Mudd, M. Quiñones, E. Schwartzman, N. R. Klatt, L. Canary, J. D. Estes, J. M. Brenchley

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Infection of gut-resident CD4 + memory T cells during acute human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection is associated with rapid loss of these cells and damage to the epithelial barrier. Damage to the epithelial barrier allows translocation of microbial products from the intestinal lumen into the body. Immune activation caused by these microbial products has been associated with disease progression. Although microbial translocation has been demonstrated in SIV-infected nonhuman primates, the identity of translocating bacteria has not been determined. In this study we examined the communities of bacteria both within the gastrointestinal (GI) tract and systemic tissues of both healthy and experimentally SIV-infected Asian macaques. Although there were only modest changes in the GI tract-associated microbiome resulting from infection, there is substantial dysbiosis after administration of antiretrovirals. Analysis of bacterial DNA isolated from tissues of infected animals revealed a preference for the phylum Proteobacteria, suggesting that they preferentially translocate. Consistent with this finding, we observed increased metabolic activity of Proteobacterial species within the colonic lumen of SIV-infected animals. Overall, these data provide insights into disease progression and suggest that therapies aimed at altering the composition and metabolic activity of the GI tract microbiome could benefit chronically HIV-infected individuals, particularly those on antiretroviral therapies.

Original languageEnglish (US)
Pages (from-to)1009-1020
Number of pages12
JournalMucosal Immunology
Volume8
Issue number5
DOIs
StatePublished - Sep 19 2015

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Dysbiotic bacteria translocate in progressive SIV infection'. Together they form a unique fingerprint.

  • Cite this

    Klase, Z., Ortiz, A., Deleage, C., Mudd, J. C., Quiñones, M., Schwartzman, E., Klatt, N. R., Canary, L., Estes, J. D., & Brenchley, J. M. (2015). Dysbiotic bacteria translocate in progressive SIV infection. Mucosal Immunology, 8(5), 1009-1020. https://doi.org/10.1038/mi.2014.128