Dysbiotic bacteria translocate in progressive SIV infection

Z. Klase, A. Ortiz, C. Deleage, J. C. Mudd, M. Quiñones, E. Schwartzman, N. R. Klatt, L. Canary, Jacob Estes, J. M. Brenchley

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Infection of gut-resident CD4 + memory T cells during acute human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) infection is associated with rapid loss of these cells and damage to the epithelial barrier. Damage to the epithelial barrier allows translocation of microbial products from the intestinal lumen into the body. Immune activation caused by these microbial products has been associated with disease progression. Although microbial translocation has been demonstrated in SIV-infected nonhuman primates, the identity of translocating bacteria has not been determined. In this study we examined the communities of bacteria both within the gastrointestinal (GI) tract and systemic tissues of both healthy and experimentally SIV-infected Asian macaques. Although there were only modest changes in the GI tract-associated microbiome resulting from infection, there is substantial dysbiosis after administration of antiretrovirals. Analysis of bacterial DNA isolated from tissues of infected animals revealed a preference for the phylum Proteobacteria, suggesting that they preferentially translocate. Consistent with this finding, we observed increased metabolic activity of Proteobacterial species within the colonic lumen of SIV-infected animals. Overall, these data provide insights into disease progression and suggest that therapies aimed at altering the composition and metabolic activity of the GI tract microbiome could benefit chronically HIV-infected individuals, particularly those on antiretroviral therapies.

Original languageEnglish (US)
Pages (from-to)1009-1020
Number of pages12
JournalMucosal Immunology
Volume8
Issue number5
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Simian Immunodeficiency Virus
Virus Diseases
Bacteria
Gastrointestinal Tract
Disease Progression
Dysbiosis
HIV
Proteobacteria
Bacterial DNA
Microbiota
Macaca
Infection
Primates
T-Lymphocytes
Therapeutics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Klase, Z., Ortiz, A., Deleage, C., Mudd, J. C., Quiñones, M., Schwartzman, E., ... Brenchley, J. M. (2015). Dysbiotic bacteria translocate in progressive SIV infection. Mucosal Immunology, 8(5), 1009-1020. https://doi.org/10.1038/mi.2014.128

Dysbiotic bacteria translocate in progressive SIV infection. / Klase, Z.; Ortiz, A.; Deleage, C.; Mudd, J. C.; Quiñones, M.; Schwartzman, E.; Klatt, N. R.; Canary, L.; Estes, Jacob; Brenchley, J. M.

In: Mucosal Immunology, Vol. 8, No. 5, 01.01.2015, p. 1009-1020.

Research output: Contribution to journalArticle

Klase, Z, Ortiz, A, Deleage, C, Mudd, JC, Quiñones, M, Schwartzman, E, Klatt, NR, Canary, L, Estes, J & Brenchley, JM 2015, 'Dysbiotic bacteria translocate in progressive SIV infection', Mucosal Immunology, vol. 8, no. 5, pp. 1009-1020. https://doi.org/10.1038/mi.2014.128
Klase Z, Ortiz A, Deleage C, Mudd JC, Quiñones M, Schwartzman E et al. Dysbiotic bacteria translocate in progressive SIV infection. Mucosal Immunology. 2015 Jan 1;8(5):1009-1020. https://doi.org/10.1038/mi.2014.128
Klase, Z. ; Ortiz, A. ; Deleage, C. ; Mudd, J. C. ; Quiñones, M. ; Schwartzman, E. ; Klatt, N. R. ; Canary, L. ; Estes, Jacob ; Brenchley, J. M. / Dysbiotic bacteria translocate in progressive SIV infection. In: Mucosal Immunology. 2015 ; Vol. 8, No. 5. pp. 1009-1020.
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