Dynorphin-mediated antinociceptive effects of l-arginine and SIN-1 (an NO donor) in mice

Eunhee Chung, Brianna Burke, Andrew Bieber, Jason C. Doss, Yusuke Ohgami, Raymond M. Quock

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The antinociceptive response of mice to the amino acid l-arginine (l-ARG) has been attributed to either an opioid mechanism or a non-opioid but nitric oxide (NO)-dependent mechanism. Earlier it was reported that the mechanism of nitrous oxide-induced antinociception involved opioid components and was also dependent on brain NO. This study was designed to determine whether the antinociceptive effects of l-ARG and the NO donor 3-morpholinosydnoimine (SIN-1) might be mediated by brain mechanisms similar to those that are responsible for nitrous oxide (N2O) antinociception. l-ARG and SIN-1 were administered to mice intracerebroventricularly (i.c.v.), and antinociception was assessed using the acetic acid abdominal constriction test. Both l-ARG and SIN-1 caused dose-related antinociceptive effects that were blocked by naloxone and norbinaltorphimine. The antinociceptive effects of both SIN-1 and l-ARG were also blocked to a greater extent by i.c.v. administration of a rabbit antiserum against rat dynorphin 1-13 than an antiserum against methionine-enkephalin, suggesting that the SIN-1 and l-ARG effects may be related to stimulated release of dynorphin. The antinociceptive effect of l-ARG was antagonized by an inhibitor of neuoronal NO synthase enzyme, indicating that l-ARG had to be converted to NO for its antinociceptive action. These findings indicate that the mechanisms of antinociceptive action of l-ARG and SIN-1 are both mediated by dynorphin and dependent on NO.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalBrain Research Bulletin
Volume70
Issue number3
DOIs
StatePublished - Jul 31 2006
Externally publishedYes

Fingerprint

Dynorphins
Nitric Oxide Donors
Arginine
Nitric Oxide
Nitrous Oxide
Opioid Analgesics
Immune Sera
Methionine Enkephalin
Brain
Naloxone
Constriction
Nitric Oxide Synthase
Acetic Acid
Rabbits
Amino Acids
Enzymes

Keywords

  • Antinociception
  • Arginine
  • Dynorphin
  • Mice
  • Nitric oxide
  • NO donors
  • Opioid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dynorphin-mediated antinociceptive effects of l-arginine and SIN-1 (an NO donor) in mice. / Chung, Eunhee; Burke, Brianna; Bieber, Andrew; Doss, Jason C.; Ohgami, Yusuke; Quock, Raymond M.

In: Brain Research Bulletin, Vol. 70, No. 3, 31.07.2006, p. 245-250.

Research output: Contribution to journalArticle

Chung, Eunhee ; Burke, Brianna ; Bieber, Andrew ; Doss, Jason C. ; Ohgami, Yusuke ; Quock, Raymond M. / Dynorphin-mediated antinociceptive effects of l-arginine and SIN-1 (an NO donor) in mice. In: Brain Research Bulletin. 2006 ; Vol. 70, No. 3. pp. 245-250.
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