Dynamics of the transcriptome in the primate ovulatory follicle

Fuhua Xu, Richard Stouffer, Jörg Müller, Jon Hennebold, Jay W. Wright, Alistair Bahar, Gabriele Leder, Michaele Peters, Melissa Thorne, Micaela Sims, Tim Wintermantel, Bernhard Lindenthal

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    Experiments were designed to evaluate changes in the transcriptome (mRNA levels) in the ovulatory, luteinizing follicle of rhesus monkeys, using a controlled ovulation model that permits analysis of the naturally selected, dominant follicle at specific intervals (0, 12, 24 and 36 h) after exposure to an ovulatory (exogenous hCG) stimulus during the menstrual cycle. Total RNA was prepared from individual follicles (n = 4-8/timepoint), with an aliquot used for microarray analysis (AffymetrixTM Rhesus Macaque Genome Array) and the remainder applied to quantitative real-time PCR (q-PCR) assays. The microarray data from individual samples distinctly clustered according to timepoints, and ovulated follicles displayed markedly different expression patterns from unruptured follicles at 36 h. Between timepoint comparisons revealed profound changes in mRNA expression profiles. The dynamic pattern of mRNA expression for steroidogenic enzymes (CYP17A, CYP19A, HSD3B2, HSD11B1 and HSD11B2), steroidogenic acute regulatory protein (StAR) and gonadotrophin receptors [LH/choriogonadotrophin receptor (LHCGR), FSH receptor (FSHR)] as determined by microarray analysis correlated precisely with those from blinded q-PCR assays. Patterns of mRNA expression for epidermal-growth-factor-like factors (amphiregulin, epiregulin) and processes [hyaluronan synthase 2 (HAS2), tumor necrosis factor alpha-induced protein 6 (TNFAIP6)] implicated in cumulus-oocyte maturation/expansion were also comparable between assays. Thus, several mRNAs displayed the expected expression pattern for purported theca (e.g. CYP17A), granulosa (CYP19A, FSHR), cumulus (HAS2, TNFAIP6) cell and surface epithelium (HSD11B)-related genes in the rodent/primate pre-ovulatory follicle. This database will be of great value in analyzing molecular and cellular pathways associated with periovulatory events in the primate follicle (e.g. follicle rupture, luteinization, inflammatory response and angiogenesis), and for identifying novel gene products controlling mammalian fertility.

    Original languageEnglish (US)
    Article numbergaq089
    Pages (from-to)152-165
    Number of pages14
    JournalMolecular Human Reproduction
    Volume17
    Issue number3
    DOIs
    StatePublished - Mar 2011

    Fingerprint

    Transcriptome
    Primates
    Messenger RNA
    FSH Receptors
    Microarray Analysis
    Macaca mulatta
    Real-Time Polymerase Chain Reaction
    Tumor Necrosis Factor-alpha
    Gonadotropin Receptors
    Luteinization
    LH Receptors
    Chorionic Gonadotropin
    Menstrual Cycle
    Ovulation
    Epidermal Growth Factor
    Genes
    Oocytes
    Fertility
    Rupture
    Rodentia

    Keywords

    • Cumulus expansion
    • Luteinization
    • Oocyte maturation
    • Ovulation
    • Rhesus monkey

    ASJC Scopus subject areas

    • Molecular Biology
    • Embryology
    • Cell Biology
    • Genetics
    • Developmental Biology
    • Reproductive Medicine
    • Obstetrics and Gynecology

    Cite this

    Dynamics of the transcriptome in the primate ovulatory follicle. / Xu, Fuhua; Stouffer, Richard; Müller, Jörg; Hennebold, Jon; Wright, Jay W.; Bahar, Alistair; Leder, Gabriele; Peters, Michaele; Thorne, Melissa; Sims, Micaela; Wintermantel, Tim; Lindenthal, Bernhard.

    In: Molecular Human Reproduction, Vol. 17, No. 3, gaq089, 03.2011, p. 152-165.

    Research output: Contribution to journalArticle

    Xu, F, Stouffer, R, Müller, J, Hennebold, J, Wright, JW, Bahar, A, Leder, G, Peters, M, Thorne, M, Sims, M, Wintermantel, T & Lindenthal, B 2011, 'Dynamics of the transcriptome in the primate ovulatory follicle', Molecular Human Reproduction, vol. 17, no. 3, gaq089, pp. 152-165. https://doi.org/10.1093/molehr/gaq089
    Xu, Fuhua ; Stouffer, Richard ; Müller, Jörg ; Hennebold, Jon ; Wright, Jay W. ; Bahar, Alistair ; Leder, Gabriele ; Peters, Michaele ; Thorne, Melissa ; Sims, Micaela ; Wintermantel, Tim ; Lindenthal, Bernhard. / Dynamics of the transcriptome in the primate ovulatory follicle. In: Molecular Human Reproduction. 2011 ; Vol. 17, No. 3. pp. 152-165.
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