Dynamics of speB mRNA transcripts in streptococcus pyogenes

Zhiyun Chen, Andreas Itzek, Horst Malke, Joseph J. Ferretti, Jens Kreth

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Streptococcus pyogenes (group A streptococcus [GAS]) is a human-specific pathogen that causes a variety of diseases ranging from superficial infections to life-threatening diseases. SpeB, a potent extracellular cysteine proteinase, plays an important role in the pathogenesis of GAS infections. Previous studies show that SpeB expression and activity are controlled at the transcriptional and posttranslational levels, though it had been unclear whether speB was also regulated at the posttranscriptional level. In this study, we examined the growth phase-dependent speB mRNA level and decay using quantitative reverse transcription-PCR (qRT-PCR) and Northern blot analyses. We observed that speB mRNA accumulated rapidly during exponential growth, which occurred concomitantly with an increase in speB mRNA stability. A closer observation revealed that the increased speB mRNA stability was mainly due to progressive acidification. Inactivation of RNase Y, a recently identified endoribonuclease, revealed a role in processing and degradation of speB mRNA. We conclude that the increased speB mRNA stability contributes to the rapid accumulation of speB transcript during growth.

Original languageEnglish (US)
Pages (from-to)1417-1426
Number of pages10
JournalJournal of Bacteriology
Volume194
Issue number6
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Streptococcus pyogenes
RNA Stability
Messenger RNA
Streptococcus
Growth
Endoribonucleases
Cysteine Proteases
Ribonucleases
Infection
Northern Blotting
Reverse Transcription
Observation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Dynamics of speB mRNA transcripts in streptococcus pyogenes. / Chen, Zhiyun; Itzek, Andreas; Malke, Horst; Ferretti, Joseph J.; Kreth, Jens.

In: Journal of Bacteriology, Vol. 194, No. 6, 01.03.2012, p. 1417-1426.

Research output: Contribution to journalArticle

Chen, Z, Itzek, A, Malke, H, Ferretti, JJ & Kreth, J 2012, 'Dynamics of speB mRNA transcripts in streptococcus pyogenes', Journal of Bacteriology, vol. 194, no. 6, pp. 1417-1426. https://doi.org/10.1128/JB.06612-11
Chen, Zhiyun ; Itzek, Andreas ; Malke, Horst ; Ferretti, Joseph J. ; Kreth, Jens. / Dynamics of speB mRNA transcripts in streptococcus pyogenes. In: Journal of Bacteriology. 2012 ; Vol. 194, No. 6. pp. 1417-1426.
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