Dynamics of Myc/Max/Mad expression during luteinization of primate granulosa cells in vitro

Association with periovulatory proliferation

Charles L. Chaffin, Rebecca S. Brogan, Richard Stouffer, Catherine A. Vandevoort

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Granulosa cell luteinization involves the attenuation of gonadotropin-induced proliferation. Although recent evidence indicates that primate granulosa cells stop dividing within 12 h of an ovulatory stimulus, early events in cell cycle arrest remain unknown. In the current study an in vitro model of primate granulosa cell luteinization is established that allows assessment of early events in terminal differentiation. A luteinizing dose of human chorionic gonadotropin (hCG) results in a secondary rise in proliferation before cell cycle arrest that is paralleled by a transient increase in the expression of c-Myc. In contrast, the c-Myc antagonists Mad1, Mad4, and Mxi1 are transiently repressed by hCG. Max, the common dimerization partner for Myc and Mad, is similarly repressed by hCG, suggesting that changes in the expression of this gene may further regulate the activity of Myc and Mad. To determine whether other cell cycle regulatory families are involved in luteinization, the expression of p53 and the wild-type p53-inducible phosphatase (wip1) was examined. Similar to Mad and Max, p53 and wip1 are transiently repressed by hCG, suggesting that the p53 and Mad pathways have either parallel or cooperative roles in luteinization. Thus, luteinization of primate granulosa cells is preceded by a burst of proliferation that is regulated by changes in the relative levels of c-Myc, Max, and Mad as well as p53.

    Original languageEnglish (US)
    Pages (from-to)1249-1256
    Number of pages8
    JournalEndocrinology
    Volume144
    Issue number4
    DOIs
    StatePublished - Apr 1 2003

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    Luteinization
    Granulosa Cells
    Primates
    Chorionic Gonadotropin
    Cell Cycle Checkpoints
    Dimerization
    Gonadotropins
    Phosphoric Monoester Hydrolases
    Cell Cycle
    In Vitro Techniques
    Gene Expression

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Dynamics of Myc/Max/Mad expression during luteinization of primate granulosa cells in vitro : Association with periovulatory proliferation. / Chaffin, Charles L.; Brogan, Rebecca S.; Stouffer, Richard; Vandevoort, Catherine A.

    In: Endocrinology, Vol. 144, No. 4, 01.04.2003, p. 1249-1256.

    Research output: Contribution to journalArticle

    Chaffin, Charles L. ; Brogan, Rebecca S. ; Stouffer, Richard ; Vandevoort, Catherine A. / Dynamics of Myc/Max/Mad expression during luteinization of primate granulosa cells in vitro : Association with periovulatory proliferation. In: Endocrinology. 2003 ; Vol. 144, No. 4. pp. 1249-1256.
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