Dynamic transport of SNARE proteins in the Golgi apparatus

Pierre Cosson, Mariella Ravazzola, Oleg Varlamov, Thomas H. Söllner, Maurizio Di Liberto, Allen Volchuk, James E. Rothman, Lelio Orci

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Localization of a membrane protein in a subcellular compartment can be achieved by its retention in the compartment or by its continuous transport toward this compartment. Previous results have suggested that specific enzymes are localized in the Golgi apparatus at least in part by selective retention and exclusion from transport vesicles. However, the function of some Golgi SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins is not compatible with their exclusion from transport vesicles. To help understand the mechanism accounting for the localization of SNARE proteins in the Golgi apparatus, we analyzed their lateral distribution in the Golgi cisternae and their incorporation into transport vesicles. According to our results, all SNARE proteins are efficiently incorporated into transport vesicles, indicating that the localization of SNARE proteins in the Golgi apparatus is not based on a static retention mechanism. Detailed analysis suggested that incorporation into transport vesicles was more efficient for SNARE proteins restricted to the cis face of the Golgi as compared with SNAREs present at the trans face. Furthermore, overexpression of a cis-Golgi SNARE protein altered concomitantly its incorporation in transport vesicles and its intra-Golgi localization. These observations suggest that, contrary to resident Golgi enzymes, SNARE proteins are localized in the Golgi apparatus as the result of a dynamic transport equilibrium.

Original languageEnglish (US)
Pages (from-to)14647-14652
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number41
DOIs
StatePublished - Oct 11 2005
Externally publishedYes

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SNARE Proteins
Golgi Apparatus
Transport Vesicles
Carrier Proteins
Enzymes
Membrane Proteins

Keywords

  • Cisternal maturation
  • Glycosyltransferase
  • Membrane sorting
  • Secretion
  • Vesicular transport

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Dynamic transport of SNARE proteins in the Golgi apparatus. / Cosson, Pierre; Ravazzola, Mariella; Varlamov, Oleg; Söllner, Thomas H.; Di Liberto, Maurizio; Volchuk, Allen; Rothman, James E.; Orci, Lelio.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 41, 11.10.2005, p. 14647-14652.

Research output: Contribution to journalArticle

Cosson, P, Ravazzola, M, Varlamov, O, Söllner, TH, Di Liberto, M, Volchuk, A, Rothman, JE & Orci, L 2005, 'Dynamic transport of SNARE proteins in the Golgi apparatus', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 41, pp. 14647-14652. https://doi.org/10.1073/pnas.0507394102
Cosson, Pierre ; Ravazzola, Mariella ; Varlamov, Oleg ; Söllner, Thomas H. ; Di Liberto, Maurizio ; Volchuk, Allen ; Rothman, James E. ; Orci, Lelio. / Dynamic transport of SNARE proteins in the Golgi apparatus. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 41. pp. 14647-14652.
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