Dynamic properties of APC-decorated microtubules in living cells

Rejith Dayanandan, Rachel Butler, Phillip R. Gordon-Weeks, Andrew Matus, Stefanie Kaech-Petrie, Simon Lovestone, Brian H. Anderton, Jean Marc Gallo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The adenomatous polyposis coli (APC) tumour suppressor protein is a component of the Wnt signalling pathway in which it plays a major role in controlling nuclear accumulation of β-catenin and hence in the modulation of β-catenin-regulated gene transcription. APC also associates with microtubules at the ends of cytoplasmic extensions in epithelial cells, a distribution that can be reproduced in COS cells ectopically expressing APC. To examine the effect of APC on microtubule properties, we monitored directly the behaviour of APC and of APC-decorated microtubules by time-lapse imaging of cytoplasmic extensions in live COS cells expressing APC tagged with a green fluorescent protein. On the proximal part of microtubules, APC was visualised as particulate material moving unidirectionally towards the plus end of microtubules. The distal parts of microtubules were uniformly decorated by APC and were animated by a motile behaviour in the form of aperiodic bending. This behaviour is likely to be the consequence of compression forces acting on microtubules encountering obstacles while elongating. The majority of APC-decorated microtubules in transfected COS cells was sensitive to depolymerisation by nocodazole, but they contained detyrosinated and acetylated α-tubulin, suggesting a reduction in the rate of subunit exchange at their growing end. Taken together, these results demonstrate that microtubule domains uniformly decorated by APC display dynamic and motile properties that may be significant for the postulated role of APC in targeting microtubules to specialised membrane sites.

Original languageEnglish (US)
Pages (from-to)237-247
Number of pages11
JournalCell Motility and the Cytoskeleton
Volume54
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Fingerprint

Adenomatous Polyposis Coli
Microtubules
COS Cells
Catenins
Adenomatous Polyposis Coli Protein
Time-Lapse Imaging
Nocodazole
Tumor Suppressor Proteins
Wnt Signaling Pathway
Tubulin
Green Fluorescent Proteins
Epithelial Cells

Keywords

  • Cytoskeleton
  • Live cell imaging
  • Microtubule dynamics
  • Microtubule-associated proteins
  • Wnt pathway

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dayanandan, R., Butler, R., Gordon-Weeks, P. R., Matus, A., Kaech-Petrie, S., Lovestone, S., ... Gallo, J. M. (2003). Dynamic properties of APC-decorated microtubules in living cells. Cell Motility and the Cytoskeleton, 54(3), 237-247. https://doi.org/10.1002/cm.10096

Dynamic properties of APC-decorated microtubules in living cells. / Dayanandan, Rejith; Butler, Rachel; Gordon-Weeks, Phillip R.; Matus, Andrew; Kaech-Petrie, Stefanie; Lovestone, Simon; Anderton, Brian H.; Gallo, Jean Marc.

In: Cell Motility and the Cytoskeleton, Vol. 54, No. 3, 01.03.2003, p. 237-247.

Research output: Contribution to journalArticle

Dayanandan, R, Butler, R, Gordon-Weeks, PR, Matus, A, Kaech-Petrie, S, Lovestone, S, Anderton, BH & Gallo, JM 2003, 'Dynamic properties of APC-decorated microtubules in living cells', Cell Motility and the Cytoskeleton, vol. 54, no. 3, pp. 237-247. https://doi.org/10.1002/cm.10096
Dayanandan R, Butler R, Gordon-Weeks PR, Matus A, Kaech-Petrie S, Lovestone S et al. Dynamic properties of APC-decorated microtubules in living cells. Cell Motility and the Cytoskeleton. 2003 Mar 1;54(3):237-247. https://doi.org/10.1002/cm.10096
Dayanandan, Rejith ; Butler, Rachel ; Gordon-Weeks, Phillip R. ; Matus, Andrew ; Kaech-Petrie, Stefanie ; Lovestone, Simon ; Anderton, Brian H. ; Gallo, Jean Marc. / Dynamic properties of APC-decorated microtubules in living cells. In: Cell Motility and the Cytoskeleton. 2003 ; Vol. 54, No. 3. pp. 237-247.
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