Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs

C. Loeber, S. J. Goldberg, M. J C Hendrix, David Sahn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ultrasonic cardiac imaging of human fetuses has been feasible only after 12 to 14 weeks gestation when the heart is completely formed. The authors' purpose was to develop a method of examining dynamic mammalian cardiogenesis with a high-resolution 9 MHz ultrasound scanner. Fetal guinea pig hearts were imaged in utero over the course of 10 days. All images were obtained after blastocyst implantation estimated to occur 8 days after fertilization with cardiogenesis occurring over the next 14 days. After laparotomies were performed on the gravid guinea pigs, sterile transducers were placed on their uterine horns and ultrasonic images were obtained. Further optical magnification of videotaped images was required to visualize cardiac details. Ten gravid guinea pigs were examined and 3 of 10 underwent serial imaging. The earliest cardiac images, recorded approximately 10 days after implantation, demonstrated a partially fused heart tube, ventricle, bulbus cordis, and first aortic arch. During the next 24 hr looping of the heart, a primitive atrium and an unfused endocardial cushion structure were observed. Midway through the first week of cardiac development, ventricular and atrial septation and development of primitive atrioventricular valve tissue were imaged. At the end of the first week of cardiac development, more distinct endocardial cushion structure and atrial and ventricular septation were noted. Additionally, a functioning foraminal flap was observed. Midway through the second week of cardiac development, spiraling and division of the bulbus cordis were complete. Scanning electron microscopic examination verified the size and configuration of ultrasonically imaged structures. The high-frequency imaging allowed dynamic recording of events in mammalian cardiogenesis.

Original languageEnglish (US)
Pages (from-to)841-845
Number of pages5
JournalCirculation
Volume68
Issue number4
StatePublished - 1983
Externally publishedYes

Fingerprint

Endocardial Cushions
Guinea Pigs
Transducers
Thoracic Aorta
Fertilization
Ultrasonics
Laparotomy
Heart Ventricles
Ultrasonography
Fetus
Electrons
Pregnancy

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Loeber, C., Goldberg, S. J., Hendrix, M. J. C., & Sahn, D. (1983). Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs. Circulation, 68(4), 841-845.

Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs. / Loeber, C.; Goldberg, S. J.; Hendrix, M. J C; Sahn, David.

In: Circulation, Vol. 68, No. 4, 1983, p. 841-845.

Research output: Contribution to journalArticle

Loeber, C, Goldberg, SJ, Hendrix, MJC & Sahn, D 1983, 'Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs', Circulation, vol. 68, no. 4, pp. 841-845.
Loeber C, Goldberg SJ, Hendrix MJC, Sahn D. Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs. Circulation. 1983;68(4):841-845.
Loeber, C. ; Goldberg, S. J. ; Hendrix, M. J C ; Sahn, David. / Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs. In: Circulation. 1983 ; Vol. 68, No. 4. pp. 841-845.
@article{2bf3917465b842839726c3744db6251d,
title = "Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs",
abstract = "Ultrasonic cardiac imaging of human fetuses has been feasible only after 12 to 14 weeks gestation when the heart is completely formed. The authors' purpose was to develop a method of examining dynamic mammalian cardiogenesis with a high-resolution 9 MHz ultrasound scanner. Fetal guinea pig hearts were imaged in utero over the course of 10 days. All images were obtained after blastocyst implantation estimated to occur 8 days after fertilization with cardiogenesis occurring over the next 14 days. After laparotomies were performed on the gravid guinea pigs, sterile transducers were placed on their uterine horns and ultrasonic images were obtained. Further optical magnification of videotaped images was required to visualize cardiac details. Ten gravid guinea pigs were examined and 3 of 10 underwent serial imaging. The earliest cardiac images, recorded approximately 10 days after implantation, demonstrated a partially fused heart tube, ventricle, bulbus cordis, and first aortic arch. During the next 24 hr looping of the heart, a primitive atrium and an unfused endocardial cushion structure were observed. Midway through the first week of cardiac development, ventricular and atrial septation and development of primitive atrioventricular valve tissue were imaged. At the end of the first week of cardiac development, more distinct endocardial cushion structure and atrial and ventricular septation were noted. Additionally, a functioning foraminal flap was observed. Midway through the second week of cardiac development, spiraling and division of the bulbus cordis were complete. Scanning electron microscopic examination verified the size and configuration of ultrasonically imaged structures. The high-frequency imaging allowed dynamic recording of events in mammalian cardiogenesis.",
author = "C. Loeber and Goldberg, {S. J.} and Hendrix, {M. J C} and David Sahn",
year = "1983",
language = "English (US)",
volume = "68",
pages = "841--845",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Dynamic mammalian cardiogenesis investigated by high-resolution ultrasound in guinea pigs

AU - Loeber, C.

AU - Goldberg, S. J.

AU - Hendrix, M. J C

AU - Sahn, David

PY - 1983

Y1 - 1983

N2 - Ultrasonic cardiac imaging of human fetuses has been feasible only after 12 to 14 weeks gestation when the heart is completely formed. The authors' purpose was to develop a method of examining dynamic mammalian cardiogenesis with a high-resolution 9 MHz ultrasound scanner. Fetal guinea pig hearts were imaged in utero over the course of 10 days. All images were obtained after blastocyst implantation estimated to occur 8 days after fertilization with cardiogenesis occurring over the next 14 days. After laparotomies were performed on the gravid guinea pigs, sterile transducers were placed on their uterine horns and ultrasonic images were obtained. Further optical magnification of videotaped images was required to visualize cardiac details. Ten gravid guinea pigs were examined and 3 of 10 underwent serial imaging. The earliest cardiac images, recorded approximately 10 days after implantation, demonstrated a partially fused heart tube, ventricle, bulbus cordis, and first aortic arch. During the next 24 hr looping of the heart, a primitive atrium and an unfused endocardial cushion structure were observed. Midway through the first week of cardiac development, ventricular and atrial septation and development of primitive atrioventricular valve tissue were imaged. At the end of the first week of cardiac development, more distinct endocardial cushion structure and atrial and ventricular septation were noted. Additionally, a functioning foraminal flap was observed. Midway through the second week of cardiac development, spiraling and division of the bulbus cordis were complete. Scanning electron microscopic examination verified the size and configuration of ultrasonically imaged structures. The high-frequency imaging allowed dynamic recording of events in mammalian cardiogenesis.

AB - Ultrasonic cardiac imaging of human fetuses has been feasible only after 12 to 14 weeks gestation when the heart is completely formed. The authors' purpose was to develop a method of examining dynamic mammalian cardiogenesis with a high-resolution 9 MHz ultrasound scanner. Fetal guinea pig hearts were imaged in utero over the course of 10 days. All images were obtained after blastocyst implantation estimated to occur 8 days after fertilization with cardiogenesis occurring over the next 14 days. After laparotomies were performed on the gravid guinea pigs, sterile transducers were placed on their uterine horns and ultrasonic images were obtained. Further optical magnification of videotaped images was required to visualize cardiac details. Ten gravid guinea pigs were examined and 3 of 10 underwent serial imaging. The earliest cardiac images, recorded approximately 10 days after implantation, demonstrated a partially fused heart tube, ventricle, bulbus cordis, and first aortic arch. During the next 24 hr looping of the heart, a primitive atrium and an unfused endocardial cushion structure were observed. Midway through the first week of cardiac development, ventricular and atrial septation and development of primitive atrioventricular valve tissue were imaged. At the end of the first week of cardiac development, more distinct endocardial cushion structure and atrial and ventricular septation were noted. Additionally, a functioning foraminal flap was observed. Midway through the second week of cardiac development, spiraling and division of the bulbus cordis were complete. Scanning electron microscopic examination verified the size and configuration of ultrasonically imaged structures. The high-frequency imaging allowed dynamic recording of events in mammalian cardiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0020566499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020566499&partnerID=8YFLogxK

M3 - Article

C2 - 6616778

AN - SCOPUS:0020566499

VL - 68

SP - 841

EP - 845

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - 4

ER -