Dynamic expression and regulation of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate ovary during the menstrual cycle

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    Abstract

    Context: Microarray analysis discovered that mRNA for CRH-binding protein (CRHBP) increased significantly in the primate corpus luteum (CL) after LH withdrawal. Objective: Our objective was to determine whether other components of the CRH/urocortin-receptor-binding protein (UCN-R-BP) system are expressed in the CL during the menstrual cycle and regulated by LH. Design: CL were collected from monkeys during the early (d 3-5 after the LH surge) to very late (d 18-19) luteal phase and from controls or animals receiving GnRH antagonist (Antide, 3 mg/kg body weight). CRH/UCN-R-BP system components were quantitated form RNA levels by real-time PCR and analyzed for protein localization by immunohistochemistry. Results: All genes encoding the CRH/UCN-R-BP system, except for UCN3, were expressed in the CL. CRH mRNA levels did not change during the luteal phase, whereas expression of UCN, UCN2, CRHR1, and CRHR2 was maximal at early or mid luteal phase before declining (P <0.05) at the later stages. CRHBP mRNA levels were lowest at mid and increased (P <0.05) in the late luteal phase. Suppressing gonadotropin secretion reduced UCN2 (P <0.05) and increased CRHBP (P <0.05) mRNA levels, without altering the expression of other ligands or receptors. CRH, UCN, UCN2 and their receptors were localized to the granulosa-lutein cells of the CL, whereas CRHBP was limited to the theca and theca-lutein cells of the preovulatory follicle and CL. Conclusions: A local CRH/UCN-R-BP system exists in the macaque CL that is dynamically expressed and LH regulated during the luteal phase of the menstrual cycle. Ligand-receptor activity may regulate luteal structure-function, at this point in an unknown manner, in primates.

    Original languageEnglish (US)
    Pages (from-to)1544-1553
    Number of pages10
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume91
    Issue number4
    DOIs
    StatePublished - Apr 2006

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    Urocortins
    Corticotropin-Releasing Hormone Receptors
    Corticotropin-Releasing Hormone
    Corpus Luteum
    Menstrual Cycle
    Primates
    Ovary
    Carrier Proteins
    Luteal Phase
    Luteal Cells
    Lutein
    Messenger RNA
    Ligands
    Gene encoding
    Macaca
    Microarray Analysis
    Microarrays
    Gonadotropins
    Gonadotropin-Releasing Hormone
    Haplorhini

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology, Diabetes and Metabolism

    Cite this

    @article{56c0635c13a345939f5a2cd0c9b55af7,
    title = "Dynamic expression and regulation of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate ovary during the menstrual cycle",
    abstract = "Context: Microarray analysis discovered that mRNA for CRH-binding protein (CRHBP) increased significantly in the primate corpus luteum (CL) after LH withdrawal. Objective: Our objective was to determine whether other components of the CRH/urocortin-receptor-binding protein (UCN-R-BP) system are expressed in the CL during the menstrual cycle and regulated by LH. Design: CL were collected from monkeys during the early (d 3-5 after the LH surge) to very late (d 18-19) luteal phase and from controls or animals receiving GnRH antagonist (Antide, 3 mg/kg body weight). CRH/UCN-R-BP system components were quantitated form RNA levels by real-time PCR and analyzed for protein localization by immunohistochemistry. Results: All genes encoding the CRH/UCN-R-BP system, except for UCN3, were expressed in the CL. CRH mRNA levels did not change during the luteal phase, whereas expression of UCN, UCN2, CRHR1, and CRHR2 was maximal at early or mid luteal phase before declining (P <0.05) at the later stages. CRHBP mRNA levels were lowest at mid and increased (P <0.05) in the late luteal phase. Suppressing gonadotropin secretion reduced UCN2 (P <0.05) and increased CRHBP (P <0.05) mRNA levels, without altering the expression of other ligands or receptors. CRH, UCN, UCN2 and their receptors were localized to the granulosa-lutein cells of the CL, whereas CRHBP was limited to the theca and theca-lutein cells of the preovulatory follicle and CL. Conclusions: A local CRH/UCN-R-BP system exists in the macaque CL that is dynamically expressed and LH regulated during the luteal phase of the menstrual cycle. Ligand-receptor activity may regulate luteal structure-function, at this point in an unknown manner, in primates.",
    author = "Jing Xu and Jon Hennebold and Richard Stouffer",
    year = "2006",
    month = "4",
    doi = "10.1210/jc.2005-2776",
    language = "English (US)",
    volume = "91",
    pages = "1544--1553",
    journal = "Journal of Clinical Endocrinology and Metabolism",
    issn = "0021-972X",
    publisher = "The Endocrine Society",
    number = "4",

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    TY - JOUR

    T1 - Dynamic expression and regulation of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate ovary during the menstrual cycle

    AU - Xu, Jing

    AU - Hennebold, Jon

    AU - Stouffer, Richard

    PY - 2006/4

    Y1 - 2006/4

    N2 - Context: Microarray analysis discovered that mRNA for CRH-binding protein (CRHBP) increased significantly in the primate corpus luteum (CL) after LH withdrawal. Objective: Our objective was to determine whether other components of the CRH/urocortin-receptor-binding protein (UCN-R-BP) system are expressed in the CL during the menstrual cycle and regulated by LH. Design: CL were collected from monkeys during the early (d 3-5 after the LH surge) to very late (d 18-19) luteal phase and from controls or animals receiving GnRH antagonist (Antide, 3 mg/kg body weight). CRH/UCN-R-BP system components were quantitated form RNA levels by real-time PCR and analyzed for protein localization by immunohistochemistry. Results: All genes encoding the CRH/UCN-R-BP system, except for UCN3, were expressed in the CL. CRH mRNA levels did not change during the luteal phase, whereas expression of UCN, UCN2, CRHR1, and CRHR2 was maximal at early or mid luteal phase before declining (P <0.05) at the later stages. CRHBP mRNA levels were lowest at mid and increased (P <0.05) in the late luteal phase. Suppressing gonadotropin secretion reduced UCN2 (P <0.05) and increased CRHBP (P <0.05) mRNA levels, without altering the expression of other ligands or receptors. CRH, UCN, UCN2 and their receptors were localized to the granulosa-lutein cells of the CL, whereas CRHBP was limited to the theca and theca-lutein cells of the preovulatory follicle and CL. Conclusions: A local CRH/UCN-R-BP system exists in the macaque CL that is dynamically expressed and LH regulated during the luteal phase of the menstrual cycle. Ligand-receptor activity may regulate luteal structure-function, at this point in an unknown manner, in primates.

    AB - Context: Microarray analysis discovered that mRNA for CRH-binding protein (CRHBP) increased significantly in the primate corpus luteum (CL) after LH withdrawal. Objective: Our objective was to determine whether other components of the CRH/urocortin-receptor-binding protein (UCN-R-BP) system are expressed in the CL during the menstrual cycle and regulated by LH. Design: CL were collected from monkeys during the early (d 3-5 after the LH surge) to very late (d 18-19) luteal phase and from controls or animals receiving GnRH antagonist (Antide, 3 mg/kg body weight). CRH/UCN-R-BP system components were quantitated form RNA levels by real-time PCR and analyzed for protein localization by immunohistochemistry. Results: All genes encoding the CRH/UCN-R-BP system, except for UCN3, were expressed in the CL. CRH mRNA levels did not change during the luteal phase, whereas expression of UCN, UCN2, CRHR1, and CRHR2 was maximal at early or mid luteal phase before declining (P <0.05) at the later stages. CRHBP mRNA levels were lowest at mid and increased (P <0.05) in the late luteal phase. Suppressing gonadotropin secretion reduced UCN2 (P <0.05) and increased CRHBP (P <0.05) mRNA levels, without altering the expression of other ligands or receptors. CRH, UCN, UCN2 and their receptors were localized to the granulosa-lutein cells of the CL, whereas CRHBP was limited to the theca and theca-lutein cells of the preovulatory follicle and CL. Conclusions: A local CRH/UCN-R-BP system exists in the macaque CL that is dynamically expressed and LH regulated during the luteal phase of the menstrual cycle. Ligand-receptor activity may regulate luteal structure-function, at this point in an unknown manner, in primates.

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    U2 - 10.1210/jc.2005-2776

    DO - 10.1210/jc.2005-2776

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    JO - Journal of Clinical Endocrinology and Metabolism

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