Dynactin-dependent, dynein-driven vesicle transport in the absence of membrane proteins: A role for spectrin and acidic phospholipids

Virgil Muresan, Michael C. Stankewich, Walter Steffen, Jon S. Morrow, Erika L F Holzbaur, Bruce Schnapp

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

We reconstituted dynein-driven, dynactin-dependent vesicle transport using protein-free liposomes and soluble components from squid axoplasm. Dynein and dynactin, while necessary, are not the only essential cytosolic factors; axonal spectrin is also required. Spectrin is resident on axonal vesicles, and rebinds from cytosol to liposomes or proteolysed vesicles, concomitant with their dynein-dynactin-dependent motility. Binding of purified axonal spectrin to liposomes requires acidic phospholipids, as does motility. Using dominant negative spectrin polypeptides and a drug that releases PH domains from membranes, we show that spectrin is required for linking dynactin, and thereby dynein, to acidic phospholipids in the membrane. We verify this model in the context of liposomes, isolated axonal vesicles, and whole axoplasm. We conclude that spectrin has an essential role in retrograde axonal transport.

Original languageEnglish (US)
Pages (from-to)173-183
Number of pages11
JournalMolecular Cell
Volume7
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

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Dyneins
Transport Vesicles
Spectrin
Staphylococcal Protein A
Phospholipids
Membrane Proteins
Liposomes
Decapodiformes
Axonal Transport
Membranes
Cytosol
Dynactin Complex
Carrier Proteins
Peptides

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Dynactin-dependent, dynein-driven vesicle transport in the absence of membrane proteins : A role for spectrin and acidic phospholipids. / Muresan, Virgil; Stankewich, Michael C.; Steffen, Walter; Morrow, Jon S.; Holzbaur, Erika L F; Schnapp, Bruce.

In: Molecular Cell, Vol. 7, No. 1, 2001, p. 173-183.

Research output: Contribution to journalArticle

Muresan, Virgil ; Stankewich, Michael C. ; Steffen, Walter ; Morrow, Jon S. ; Holzbaur, Erika L F ; Schnapp, Bruce. / Dynactin-dependent, dynein-driven vesicle transport in the absence of membrane proteins : A role for spectrin and acidic phospholipids. In: Molecular Cell. 2001 ; Vol. 7, No. 1. pp. 173-183.
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