Dual regulation of insulin-like growth factor I expression during renal hypertrophy

R. Lajara, P. Rotwein, J. D. Bortz, V. A. Hansen, J. L. Sadow, C. R. Betts, S. A. Rogers, M. R. Hammerman

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Abstract

We examined the regulation of insulin-like growth factor I (IGF-I) in kidney during the renal hypertrophy produced by two different experimental models: growth hormone treatment of hypophysectomized rats and compensatory hypertrophy subsequent to unilateral nephrectomy. Immunostaining for IGF-I in collecting ducts was enhanced in kidneys from growth hormone-repleted hypophysectomized rats, and the levels of IGF-I mRNAs were increased. In compensatory hypertrophy, no enhancement of the intensity of immunostaining was observed in kidneys of nephrectomized rats until 5 days postnephrectomy, at which time immunostainable IGF-I was increased markedly in medullary collecting ducts of hypertrophied kidneys compared with kidneys from sham-operated animals. No difference in steady-state levels of any IGF-I mRNA species was detected in whole kidneys or in collecting ducts from nephrectomized or sham-operated rats at any time postnephrectomy. Our findings demonstrate an increase in both IGF-I mRNA and in immunostainable IGF-I in collecting duct in the setting of growth hormone-induced renal hypertrophy but suggest that other, possibly translational, mechanisms underlie the induction of IGF-I synthesis during compensatory hypertrophy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume257
Issue number2
StatePublished - 1989
Externally publishedYes

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Insulin-Like Growth Factor I
Hypertrophy
Kidney
Growth Hormone
Messenger RNA
Collecting Kidney Tubules
Nephrectomy
Theoretical Models

ASJC Scopus subject areas

  • Physiology

Cite this

Lajara, R., Rotwein, P., Bortz, J. D., Hansen, V. A., Sadow, J. L., Betts, C. R., ... Hammerman, M. R. (1989). Dual regulation of insulin-like growth factor I expression during renal hypertrophy. American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 257(2).

Dual regulation of insulin-like growth factor I expression during renal hypertrophy. / Lajara, R.; Rotwein, P.; Bortz, J. D.; Hansen, V. A.; Sadow, J. L.; Betts, C. R.; Rogers, S. A.; Hammerman, M. R.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 257, No. 2, 1989.

Research output: Contribution to journalArticle

Lajara, R, Rotwein, P, Bortz, JD, Hansen, VA, Sadow, JL, Betts, CR, Rogers, SA & Hammerman, MR 1989, 'Dual regulation of insulin-like growth factor I expression during renal hypertrophy', American Journal of Physiology - Renal Fluid and Electrolyte Physiology, vol. 257, no. 2.
Lajara, R. ; Rotwein, P. ; Bortz, J. D. ; Hansen, V. A. ; Sadow, J. L. ; Betts, C. R. ; Rogers, S. A. ; Hammerman, M. R. / Dual regulation of insulin-like growth factor I expression during renal hypertrophy. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1989 ; Vol. 257, No. 2.
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