D2-like dopamine receptors promote interactions between calcium and chloride channels that diminish rod synaptic transfer in the salamander retina

Wallace B. Thoreson, Salvatore L. Stella, Eric J. Bryson, John Clements, Paul Witkovsky

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Activation of D2-like dopamine receptors in rods with quinpirole stimulates L-type calcium currents (ICa). This result appears inconsistent with studies showing that D2-like dopamine receptor activation diminishes rod signals in second-order retinal neurons. Since small reductions in [Cl-]i can inhibit photoreceptor ICa, we tested the hypothesis that enhancement of ICa, with the D2/D4 receptor agonist, quinpirole, increases calcium-activated chloride currents (ICl(Ca)) causing an efflux of Cl- from rods that would provide a negative feedback inhibition of ICa. In agreement with studies from Xenopus, quinpirole reduced rod input to second-order neurons of tiger salamander retina without significantly altering rod voltage responses. Quinpirole also diminished the amplitude of depolarization-evoked increases in [Ca2+], measured with Fura-2 in rods, a finding consistent with inhibition of synaptic transmission from rods. Electrophysiological and Cl--imaging experiments indicated ECl in rods is ∼ -20 mV. Quinpirole enhanced ICl(Ca) and elicited an efflux of Cl- at the resting potential. A similar Cl- efflux was produced by extracellular replacement of 24 mM Cl- with CH3SO4- and this low Cl- solution inhibited Ca2+responses to a similar degree as quinpirole did. When ICl(Ca) was inhibited with niflumic acid, quinpirole enhanced both ICa and depolarization-evoked increases in [Ca2+]i. Furthermore, with niflumic acid, quinpirole no longer inhibited rod inputs into horizontal and bipolar cells. These results suggest an initial enhancement of ICa by quinpirole is followed by a stimulation of Cl- currents, including ICl(Ca). The net result is a Cl- efflux that inhibits depolarization-evoked increases in [Ca2+]i and synaptic transmission from rods.

Original languageEnglish (US)
Pages (from-to)235-247
Number of pages13
JournalVisual Neuroscience
Volume19
Issue number3
DOIs
StatePublished - May 2002
Externally publishedYes

Fingerprint

Quinpirole
Urodela
Calcium Chloride
Chloride Channels
Dopamine D2 Receptors
Calcium Channels
Retina
Niflumic Acid
Synaptic Transmission
Ambystoma
Retinal Neurons
Fura-2
Xenopus
Membrane Potentials
Calcium
Neurons

Keywords

  • Calcium current
  • Calcium-activated chloride current
  • Electrophysiology
  • Imaging
  • Outer retina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

D2-like dopamine receptors promote interactions between calcium and chloride channels that diminish rod synaptic transfer in the salamander retina. / Thoreson, Wallace B.; Stella, Salvatore L.; Bryson, Eric J.; Clements, John; Witkovsky, Paul.

In: Visual Neuroscience, Vol. 19, No. 3, 05.2002, p. 235-247.

Research output: Contribution to journalArticle

Thoreson, Wallace B. ; Stella, Salvatore L. ; Bryson, Eric J. ; Clements, John ; Witkovsky, Paul. / D2-like dopamine receptors promote interactions between calcium and chloride channels that diminish rod synaptic transfer in the salamander retina. In: Visual Neuroscience. 2002 ; Vol. 19, No. 3. pp. 235-247.
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abstract = "Activation of D2-like dopamine receptors in rods with quinpirole stimulates L-type calcium currents (ICa). This result appears inconsistent with studies showing that D2-like dopamine receptor activation diminishes rod signals in second-order retinal neurons. Since small reductions in [Cl-]i can inhibit photoreceptor ICa, we tested the hypothesis that enhancement of ICa, with the D2/D4 receptor agonist, quinpirole, increases calcium-activated chloride currents (ICl(Ca)) causing an efflux of Cl- from rods that would provide a negative feedback inhibition of ICa. In agreement with studies from Xenopus, quinpirole reduced rod input to second-order neurons of tiger salamander retina without significantly altering rod voltage responses. Quinpirole also diminished the amplitude of depolarization-evoked increases in [Ca2+], measured with Fura-2 in rods, a finding consistent with inhibition of synaptic transmission from rods. Electrophysiological and Cl--imaging experiments indicated ECl in rods is ∼ -20 mV. Quinpirole enhanced ICl(Ca) and elicited an efflux of Cl- at the resting potential. A similar Cl- efflux was produced by extracellular replacement of 24 mM Cl- with CH3SO4- and this low Cl- solution inhibited Ca2+responses to a similar degree as quinpirole did. When ICl(Ca) was inhibited with niflumic acid, quinpirole enhanced both ICa and depolarization-evoked increases in [Ca2+]i. Furthermore, with niflumic acid, quinpirole no longer inhibited rod inputs into horizontal and bipolar cells. These results suggest an initial enhancement of ICa by quinpirole is followed by a stimulation of Cl- currents, including ICl(Ca). The net result is a Cl- efflux that inhibits depolarization-evoked increases in [Ca2+]i and synaptic transmission from rods.",
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