dSLo interacting protein 1, a novel protein that interacts with large- conductance calcium-activated potassium channels

Xiao Ming Xia, Birgit Hirschberg, Sarah Smolik, Michael Forte, John Adelman

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Large-conductance calcium-activated potassium channels (BK channels) are activated by depolarized membrane potential and elevated levels of intracellular calcium. BK channel activity underlies the fast after hyperpolarization that follows an action potential and attenuates neurotransmitter and hormone secretion. Using a modified two-hybrid approach, the interaction trap, we have identified a novel protein from Drosophila, dSLIP1 (dSLo interacting protein), which specifically interacts with Drosophila and human BK channels and has partial homology to the PDZ domain of α1 syntrophin. The dSLIP1 and dSlo mRNAs are expressed coincidently throughout the Drosophila nervous system, the two proteins interact in vitro, and they may be coimmunoprecipitated from transfected cells. Coexpression of dSLIP1 with dSlo or hSlo BK channels in Xenopus oocytes results in reduced currents as compared with expression of BK channels alone; current amplitudes may be rescued by coexpression with the channel domain that interacts with dSLIP1. Single-channel recordings and immunostaining of transfected tissue culture cells suggest that dSLIP1 selectively reduces Slo BK currents by reducing the number of BK channels in the plasma membrane.

Original languageEnglish (US)
Pages (from-to)2360-2369
Number of pages10
JournalJournal of Neuroscience
Volume18
Issue number7
StatePublished - Apr 1 1998

Fingerprint

Large-Conductance Calcium-Activated Potassium Channels
Proteins
Drosophila
PDZ Domains
Xenopus
Membrane Potentials
Nervous System
Action Potentials
Oocytes
Neurotransmitter Agents
Cell Culture Techniques
Cell Membrane
Hormones
Calcium
Messenger RNA

Keywords

  • BK channels
  • Chaperone protein
  • Current density
  • Interacting protein
  • Regulated expression
  • Two-hybrid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

dSLo interacting protein 1, a novel protein that interacts with large- conductance calcium-activated potassium channels. / Xia, Xiao Ming; Hirschberg, Birgit; Smolik, Sarah; Forte, Michael; Adelman, John.

In: Journal of Neuroscience, Vol. 18, No. 7, 01.04.1998, p. 2360-2369.

Research output: Contribution to journalArticle

Xia, Xiao Ming ; Hirschberg, Birgit ; Smolik, Sarah ; Forte, Michael ; Adelman, John. / dSLo interacting protein 1, a novel protein that interacts with large- conductance calcium-activated potassium channels. In: Journal of Neuroscience. 1998 ; Vol. 18, No. 7. pp. 2360-2369.
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