TY - JOUR
T1 - Drosophila stimulatory G protein α subunit activates mammalian adenylyl cyclase but interacts poorly with mammalian receptors
T2 - Implications for receptor-G protein interaction
AU - Quan, Franklin
AU - Thomas, Laurel
AU - Forte, Michael
PY - 1991
Y1 - 1991
N2 - Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce signals from cell-surface receptors to intracellular effector proteins. Two forms of stimulatory G protein (Gs) α-like subunit have been described in Drosophila melanogaster. To examine the function of these subunits we have used vaccinia virus vectors to express both proteins in cyc- cells, a murine S49 cell line deficient for Gsα activity. Receptor-independent activation of each Drosophila Gsα has demonstrated that both forms are capable of activating mammalian adenylyl cyclase and thus have the activity expected of stimulatory G proteins. However, the Drosophila Gsα subunits interact poorly with mammalian Gs-coupled receptors. These observations have helped to identify a region of high variability in Gsα proteins that may be important for receptor interactions.
AB - Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce signals from cell-surface receptors to intracellular effector proteins. Two forms of stimulatory G protein (Gs) α-like subunit have been described in Drosophila melanogaster. To examine the function of these subunits we have used vaccinia virus vectors to express both proteins in cyc- cells, a murine S49 cell line deficient for Gsα activity. Receptor-independent activation of each Drosophila Gsα has demonstrated that both forms are capable of activating mammalian adenylyl cyclase and thus have the activity expected of stimulatory G proteins. However, the Drosophila Gsα subunits interact poorly with mammalian Gs-coupled receptors. These observations have helped to identify a region of high variability in Gsα proteins that may be important for receptor interactions.
KW - Vaccinia virus expression
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U2 - 10.1073/pnas.88.5.1898
DO - 10.1073/pnas.88.5.1898
M3 - Article
C2 - 1848015
AN - SCOPUS:0026087306
SN - 0027-8424
VL - 88
SP - 1898
EP - 1902
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 5
ER -