Drosophila stimulatory G protein α subunit activates mammalian adenylyl cyclase but interacts poorly with mammalian receptors: Implications for receptor-G protein interaction

Franklin Quan, Laurel Thomas, Michael Forte

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Abstract

Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce signals from cell-surface receptors to intracellular effector proteins. Two forms of stimulatory G protein (Gs) α-like subunit have been described in Drosophila melanogaster. To examine the function of these subunits we have used vaccinia virus vectors to express both proteins in cyc- cells, a murine S49 cell line deficient for Gsα activity. Receptor-independent activation of each Drosophila Gsα has demonstrated that both forms are capable of activating mammalian adenylyl cyclase and thus have the activity expected of stimulatory G proteins. However, the Drosophila Gsα subunits interact poorly with mammalian Gs-coupled receptors. These observations have helped to identify a region of high variability in Gsα proteins that may be important for receptor interactions.

Original languageEnglish (US)
Pages (from-to)1898-1902
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number5
DOIs
StatePublished - Jan 1 1991

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Keywords

  • Vaccinia virus expression

ASJC Scopus subject areas

  • General

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