TY - JOUR
T1 - Drosophila host defense after oral infection by an entomopathogenic Pseudomonas species
AU - Vodovar, Nicolas
AU - Vinals, Marisa
AU - Liehl, Peter
AU - Basset, Alan
AU - Degrouard, Jeril
AU - Spellman, Paul
AU - Boccard, Frédéric
AU - Lemaitre, Bruno
PY - 2005/8/9
Y1 - 2005/8/9
N2 - Drosophila has been shown to be a valuable model for the investigation of host-pathogen interactions. Study of the Drosophila immune response has been hampered, however, by the lack of true Drosophila pathogens. In nearly all studies reported, the bacteria used were directly injected within the body cavity of the insect, bypassing the initial steps of a natural interaction. Here, we report the identification of a previously uncharacterized bacterial species, Pseudomonas entomophila (Pe), which has the capacity to induce the systemic expression of antimicrobial peptide genes in Drosophila after ingestion. In contrast to previously identified bacteria, Pe is highly pathogenic to both Drosophila larvae and adults, and its persistence in larvae leads to a massive destruction of gut cells. Using this strain, we have analyzed the modulation of the larval transcriptome upon bacterial infection. We found that natural infection by Pe induces a dramatic change in larval gene expression. In addition to immunity genes, our study identifies many genes associated with Pe pathogenesis that have been previously unreported.
AB - Drosophila has been shown to be a valuable model for the investigation of host-pathogen interactions. Study of the Drosophila immune response has been hampered, however, by the lack of true Drosophila pathogens. In nearly all studies reported, the bacteria used were directly injected within the body cavity of the insect, bypassing the initial steps of a natural interaction. Here, we report the identification of a previously uncharacterized bacterial species, Pseudomonas entomophila (Pe), which has the capacity to induce the systemic expression of antimicrobial peptide genes in Drosophila after ingestion. In contrast to previously identified bacteria, Pe is highly pathogenic to both Drosophila larvae and adults, and its persistence in larvae leads to a massive destruction of gut cells. Using this strain, we have analyzed the modulation of the larval transcriptome upon bacterial infection. We found that natural infection by Pe induces a dramatic change in larval gene expression. In addition to immunity genes, our study identifies many genes associated with Pe pathogenesis that have been previously unreported.
KW - Host-microbe interaction
KW - Innate immunity
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=23844452699&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23844452699&partnerID=8YFLogxK
U2 - 10.1073/pnas.0502240102
DO - 10.1073/pnas.0502240102
M3 - Article
C2 - 16061818
AN - SCOPUS:23844452699
SN - 0027-8424
VL - 102
SP - 11414
EP - 11419
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 32
ER -