Downregulation of CCR5 on activated CD4 T cells in HIV-infected Indians

Shweta Dubey, Mohammad Khalid, Clement Wesley, Samir Ali Khan, Ajay Wanchu, Shahid Jameel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: HIV infection in India is unique as it occurs predominantly by CCR5-utilizing isolates that exhibit no co-receptor switch. Objectives: To study HIV-1 co-receptor dynamics on T cells and monocytes following viral infection. Study design: HIV co-receptor expression was evaluated by flow cytometry on various cell subsets in HIV-infected Indians and in vitro in human peripheral blood mononuclear cells infected with CCR5- or CXCR4-utilizing HIV-1. Transfection of the T cell line CEM-CCR5 (which expresses CD4, CCR5 and CXCR4) with HIV-1 Nef or Vpu expression vectors, or treatment with recombinant soluble gp120 from CCR5- and CXCR4-tropic HIV-1, was carried out to determine their effects on co-receptor expression. Results: Indian HIV patients had fewer CD4+CCR5+ T cells and CCR5-expressing activated CD4+ T cells, but higher CXCR4-expressing activated CD4+ T cells compared with controls. Expression of CCR5 was not different on monocytes in HIV patients as compared to controls. The CCR5 downregulation on T cells was HIV infection specific and was governed by the co-receptor-utilization phenotype of the virus. The Nef and soluble gp120 proteins induced CCR5 downregulation, the latter in a co-receptor-utilization phenotype specific manner. Conclusions: The HIV-1 co-receptor dynamics in Indian patients is distinct from western patients and depends upon the virus surface protein. We propose this to be a viral survival strategy.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalJournal of Clinical Virology
Volume43
Issue number1
DOIs
StatePublished - Sep 1 2008

Keywords

  • CCR5
  • CXCR4
  • Co-receptor downregulation
  • HIV
  • India

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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    Dubey, S., Khalid, M., Wesley, C., Khan, S. A., Wanchu, A., & Jameel, S. (2008). Downregulation of CCR5 on activated CD4 T cells in HIV-infected Indians. Journal of Clinical Virology, 43(1), 25-31. https://doi.org/10.1016/j.jcv.2008.03.026