Abstract
Background: HIV infection in India is unique as it occurs predominantly by CCR5-utilizing isolates that exhibit no co-receptor switch. Objectives: To study HIV-1 co-receptor dynamics on T cells and monocytes following viral infection. Study design: HIV co-receptor expression was evaluated by flow cytometry on various cell subsets in HIV-infected Indians and in vitro in human peripheral blood mononuclear cells infected with CCR5- or CXCR4-utilizing HIV-1. Transfection of the T cell line CEM-CCR5 (which expresses CD4, CCR5 and CXCR4) with HIV-1 Nef or Vpu expression vectors, or treatment with recombinant soluble gp120 from CCR5- and CXCR4-tropic HIV-1, was carried out to determine their effects on co-receptor expression. Results: Indian HIV patients had fewer CD4+CCR5+ T cells and CCR5-expressing activated CD4+ T cells, but higher CXCR4-expressing activated CD4+ T cells compared with controls. Expression of CCR5 was not different on monocytes in HIV patients as compared to controls. The CCR5 downregulation on T cells was HIV infection specific and was governed by the co-receptor-utilization phenotype of the virus. The Nef and soluble gp120 proteins induced CCR5 downregulation, the latter in a co-receptor-utilization phenotype specific manner. Conclusions: The HIV-1 co-receptor dynamics in Indian patients is distinct from western patients and depends upon the virus surface protein. We propose this to be a viral survival strategy.
Original language | English (US) |
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Pages (from-to) | 25-31 |
Number of pages | 7 |
Journal | Journal of Clinical Virology |
Volume | 43 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2008 |
Externally published | Yes |
Keywords
- CCR5
- CXCR4
- Co-receptor downregulation
- HIV
- India
ASJC Scopus subject areas
- Virology
- Infectious Diseases