Down-regulation of the chicken α5β1 integrin fibronectin receptor during development

John Muschler, Alan F. Horwitz

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We have characterized the diversity of the chicken β1 integrin family and studied the expression of individual receptors during development. The diversity of the β1 integrin family was investigated by affinity purifying the β1 integrins from a variety of adult and embryonic tissues. These purifications reveal the relative levels of expression and also the differential expression of the α subunits in those tissues. Monoclonal antibodies were generated against the prominent 'band 1' of the embryonic chicken integrins and used to characterize the expression of this α subunit in embryonic and adult tissues. This α subunit is shown to be the chicken homologue of human α5 fibronectin receptor. The chicken α5β1 integrin is the most prominent β1 integrin in the embryo and is expressed on the majority of cell types through the day 17 stage. The distribution of this receptor in the embryo closely parallels the distribution of its ligand, fibronectin. In adult tissues, expression of this receptor is greatly diminished relative to the expression of other α subunits. The cell type distribution is highly restricted: limited primarily to the vasculature and to connective tissue regions. These studies reveal a prominent role for the α5β1 integrin in embryonic cell types and a down-regulation of this receptor on many cell types during development.

Original languageEnglish (US)
Pages (from-to)327-337
Number of pages11
JournalDevelopment
Volume113
Issue number1
StatePublished - Sep 1991
Externally publishedYes

Fingerprint

Integrin alpha5beta1
Integrins
Chickens
Down-Regulation
Embryonic Structures
Fibronectins
Connective Tissue
Monoclonal Antibodies
Ligands

Keywords

  • Chick αβ integrin
  • Collagen
  • Fibronectin
  • Laminin
  • Monoclonal antibody
  • Receptor

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Down-regulation of the chicken α5β1 integrin fibronectin receptor during development. / Muschler, John; Horwitz, Alan F.

In: Development, Vol. 113, No. 1, 09.1991, p. 327-337.

Research output: Contribution to journalArticle

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