Down-regulation of negative acute-phase response genes by hypotonic stress in HepG2 hepatoma cells

Sophie Claeyssens, Fatima Banine, Philippe Rouet, Alain Lavoinne, Jean Philippe Salier

    Research output: Contribution to journalArticle

    6 Scopus citations

    Abstract

    An increased hepatocellular hydration state (HS) that can be induced by hypotonic stress or a high glutamine uptake modulates the transcription of given genes in liver. This could be important in the acute phase (AP) of a systemic inflammation where both HS and glutamine uptake transiently increase in liver. In HepG2 hepatoma cells cultured in conditions of hypotonic stress or a high extracellular glutamine availability, a specifically decreased expression of two human mRNAs, namely those of α1-microglobulin/bikunin precursor (AMBP) and α2-HS-glycoprotein, that are also down-regulated in liver by AP, could be seen. A functional analysis of the AMBP promoter indicated that this hypotonic stress-induced down-regulation takes place at a transcriptional level. In these experiments, the mRNA level and transcription of the glyceraldehyde-3-phosphate dehydrogenase gene that are known to be unmodified in AP did not exhibit any change. Given that hypotonic stress also up-regulates the transcription of a liver gene that is also up-regulated in AP [Meisse et al. (1998) FEBS Lett. 422, 346-348], the AP-associated increase in hepatocellular HS now appears to participate in the transcriptional control of both sets of genes that are up- or down-regulated in AP. Copyright (C) 1998 Federation of European Biochemical Societies.

    Original languageEnglish (US)
    Pages (from-to)15-18
    Number of pages4
    JournalFEBS Letters
    Volume433
    Issue number1-2
    DOIs
    StatePublished - Aug 14 1998

    Keywords

    • Acute-phase gene
    • Glutamine
    • Hepatoma cell
    • Hydration state
    • Transcriptional regulation

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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