TY - JOUR
T1 - Down-regulation of MEIS1 promotes the maturation of oxidative phosphorylation in perinatal cardiomyocytes
AU - Lindgren, Isa M.
AU - Drake, Rachel R.
AU - Chattergoon, Natasha N.
AU - Thornburg, Kent L.
N1 - Funding Information:
The authors thank Sonnet Jonker and Samantha Louey for helpful comments on the manuscript and Haeri Choi for technical support (all from Oregon Health and Science University). This research was supported by the M. Lowell Edwards Endowment, and the U.S. National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development (Grants 408 P01HD034430 and R21 HD090529 to K.L.T.). I.M.L. was supported by training grants from the Sixten Gemz?us Foundation, the Carl Tryggers Foundation, and the Sweden-America Foundation. The authors declare no conflicts of interest.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Fetal cardiomyocytes shift from glycolysis to oxidative phosphorylation around the time of birth. Myeloid ecotropic viral integration site 1 (MEIS1) is a transcription factor that promotes glycolysis in hematopoietic stem cells. We reasoned that MEIS1 could have a similar role in the developing heart. We hypothesized that suppression of MEIS1 expression in fetal sheep cardiomyocytes leads to a metabolic switch as found at birth. Expression of MEIS1 was assayed in left ventricular cardiac tissue and primary cultures of cardiomyocytes from fetal (100- and 135-d gestation, term = 145 d), neonatal, and adult sheep. Cultured cells were treated with short interfering RNA (siRNA) to suppress MEIS1. Oxygen consumption rate was assessed with the Seahorse metabolic flux analyzer, and mitochondrial activity was assessed by staining cells with MitoTracker Orange. Cardiomyocyte respiratory capacity increased with advancing age concurrently with decreased expression of MEIS1. MEIS1 suppression with siRNA increased maximal oxygen consumption in fetal cells but not in postnatal cells. Mitochondrial activity was increased and expression of glycolytic genes decreased when MEIS1 expression was suppressed. Thus, we conclude that MEIS1 is a key regulator of cardiomyocyte metabolism and that the normal down-regulation of MEIS1 with age underlies a gradual switch to oxidative metabolism.—Lindgren, I. M., Drake, R. R., Chattergoon, N. N., Thornburg, K. L. Down-regulation of MEIS1 promotes the maturation of oxidative phosphorylation in perinatal cardiomyocytes. FASEB J. 33, 7417–7426 (2019). www.fasebj.org.
AB - Fetal cardiomyocytes shift from glycolysis to oxidative phosphorylation around the time of birth. Myeloid ecotropic viral integration site 1 (MEIS1) is a transcription factor that promotes glycolysis in hematopoietic stem cells. We reasoned that MEIS1 could have a similar role in the developing heart. We hypothesized that suppression of MEIS1 expression in fetal sheep cardiomyocytes leads to a metabolic switch as found at birth. Expression of MEIS1 was assayed in left ventricular cardiac tissue and primary cultures of cardiomyocytes from fetal (100- and 135-d gestation, term = 145 d), neonatal, and adult sheep. Cultured cells were treated with short interfering RNA (siRNA) to suppress MEIS1. Oxygen consumption rate was assessed with the Seahorse metabolic flux analyzer, and mitochondrial activity was assessed by staining cells with MitoTracker Orange. Cardiomyocyte respiratory capacity increased with advancing age concurrently with decreased expression of MEIS1. MEIS1 suppression with siRNA increased maximal oxygen consumption in fetal cells but not in postnatal cells. Mitochondrial activity was increased and expression of glycolytic genes decreased when MEIS1 expression was suppressed. Thus, we conclude that MEIS1 is a key regulator of cardiomyocyte metabolism and that the normal down-regulation of MEIS1 with age underlies a gradual switch to oxidative metabolism.—Lindgren, I. M., Drake, R. R., Chattergoon, N. N., Thornburg, K. L. Down-regulation of MEIS1 promotes the maturation of oxidative phosphorylation in perinatal cardiomyocytes. FASEB J. 33, 7417–7426 (2019). www.fasebj.org.
KW - development
KW - fetal heart
KW - glycolysis
KW - metabolic shift
KW - mitochondrial respiration
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U2 - 10.1096/fj.201801330RR
DO - 10.1096/fj.201801330RR
M3 - Article
C2 - 30884246
AN - SCOPUS:85067267223
VL - 33
SP - 7417
EP - 7426
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 6
ER -