Double-strand breaks stimulate alternative mechanisms of recombination repair

Jac A. Nickoloff; Jeffrey D. Singer; Merl F. Hoekstra; Fred Heffron

doi:10.1016/0022-2836(89)90462-2

Double-strand breaks stimulate alternative mechanisms of recombination repair

Jac A. Nickoloff, Jeffrey D. Singer, Merl F. Hoekstra, Fred Heffron

Research output: Contribution to journal › Article › peer-review

113 Scopus citations

Abstract

To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.

Original language	English (US)
Pages (from-to)	527-541
Number of pages	15
Journal	Journal of molecular biology
Volume	207
Issue number	3
DOIs	https://doi.org/10.1016/0022-2836(89)90462-2
State	Published - Jun 5 1989
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(89)90462-2

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@article{08a476db0d3e4b87b266e45b2a8b129a,

title = "Double-strand breaks stimulate alternative mechanisms of recombination repair",

abstract = "To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.",

author = "Nickoloff, {Jac A.} and Singer, {Jeffrey D.} and Hoekstra, {Merl F.} and Fred Heffron",

note = "Funding Information: We are grateful to Phil Hieter and Ron Davis for strain YP52 and plasmid pSE271, Ira Herskowitz for plasmid CY58, Robert Griffith for synthesis of synthetic oligonucleotides and Pam Lawson for technical assistance. We thank Gerry Smith, Frank Stahl and his lab, H. Steven Seifert, Richard Reynolds, Mike Liskay, Jim Hicks and Ralph Keil for helpful discussions and critical review of the manuscript. This work was supported by National Institutes of Health grant ROl GM33808 to F.H. M.F.H. is a Lucille P. Markey Scholar in Biomedical Sciences.",

year = "1989",

month = jun,

day = "5",

doi = "10.1016/0022-2836(89)90462-2",

language = "English (US)",

volume = "207",

pages = "527--541",

journal = "Journal of molecular biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "3",

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TY - JOUR

T1 - Double-strand breaks stimulate alternative mechanisms of recombination repair

AU - Nickoloff, Jac A.

AU - Singer, Jeffrey D.

AU - Hoekstra, Merl F.

AU - Heffron, Fred

N1 - Funding Information: We are grateful to Phil Hieter and Ron Davis for strain YP52 and plasmid pSE271, Ira Herskowitz for plasmid CY58, Robert Griffith for synthesis of synthetic oligonucleotides and Pam Lawson for technical assistance. We thank Gerry Smith, Frank Stahl and his lab, H. Steven Seifert, Richard Reynolds, Mike Liskay, Jim Hicks and Ralph Keil for helpful discussions and critical review of the manuscript. This work was supported by National Institutes of Health grant ROl GM33808 to F.H. M.F.H. is a Lucille P. Markey Scholar in Biomedical Sciences.

PY - 1989/6/5

Y1 - 1989/6/5

N2 - To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.

AB - To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.

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U2 - 10.1016/0022-2836(89)90462-2

DO - 10.1016/0022-2836(89)90462-2

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SP - 527

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JO - Journal of molecular biology

JF - Journal of molecular biology

IS - 3

ER -

Double-strand breaks stimulate alternative mechanisms of recombination repair

Abstract

ASJC Scopus subject areas

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