Double-strand breaks stimulate alternative mechanisms of recombination repair

Jac A. Nickoloff, Jeffrey D. Singer, Merl F. Hoekstra, Fred Heffron

Research output: Contribution to journalArticlepeer-review

100 Scopus citations


To test the double-strand break repair model, we used HO nuclease to introduce double-strand breaks at several sites along a yeast chromosome containing duplicated DNA. Depending on the configuration of the double-strand break and recombining markers, different spectra of recombinant products were observed. Different repair kinetics and recombinant products were observed when a double-strand break was introduced in unique or duplicated DNA. The results of this study suggest that double-strand breaks in yeast stimulate recombination by several mechanisms, and we propose an alternative mechanism for double-strand break-induced gene conversion that does not depend on direct participation of the broken ends.

Original languageEnglish (US)
Pages (from-to)527-541
Number of pages15
JournalJournal of molecular biology
Issue number3
StatePublished - Jun 5 1989
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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