Double-negative (CD4-CD8-) lymphocytes bearing T-cell receptor α and β chains in normal human skin

V. Groh, M. Fabbi, F. Hochstenbach, Richard Maziarz, J. L. Strominger

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Double-negative (CD4-CD8-) T-cell receptor α- and β-chain (TCRαβ)-expressing lymphocytes have been identified in human skin. Two skin-derived lymphocyte cell lines (II-1 and II-2) expressing this phenotype were obtained. They each expressed full-length TCR α- and β-chain transcripts and lacked TCR γ- and δ-chain as well as CD4 and CD8 mRNAs. These polyclonal cell lines shared one predominant TCR β-chain gene rearrangement. The CD3/TCRαβ complexes were functionally competent as evidenced by their capacity to transduce activation signals resulting in cell proliferation, lymphokine secretion, and cytolytic activity. The demonstration of CD4-CD8- TCRαβ lymphocytes as a subpopulation of the skin-associated immune system should contribute to understanding the biologic potential of this cell population as well as of this organ-specific immune system.

Original languageEnglish (US)
Pages (from-to)5059-5063
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number13
StatePublished - 1989
Externally publishedYes

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T-Cell Antigen Receptor
Lymphocytes
Skin
Immune System
Cell Line
Gene Rearrangement
Lymphokines
Cell Proliferation
Phenotype
Messenger RNA
Population

ASJC Scopus subject areas

  • General
  • Genetics

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Double-negative (CD4-CD8-) lymphocytes bearing T-cell receptor α and β chains in normal human skin. / Groh, V.; Fabbi, M.; Hochstenbach, F.; Maziarz, Richard; Strominger, J. L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 13, 1989, p. 5059-5063.

Research output: Contribution to journalArticle

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AU - Maziarz, Richard

AU - Strominger, J. L.

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