Doppler optical coherence tomography of retinal circulation

Ou Tan, Yimin Wang, Ranjith K. Konduru, Xinbo Zhang, Srinivas R. Sadda, David Huang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Noncontact retinal blood flow measurements are performed with a Fourier domain optical coherence tomography (OCT) system using a circumpapillary double circular scan (CDCS) that scans around the optic nerve head at 3.40 mm and 3.75 mm diameters. The double concentric circles are performed 6 times consecutively over 2 sec. The CDCS scan is saved with Doppler shift information from which flow can be calculated. The standard clinical protocol calls for 3 CDCS scans made with the OCT beam passing through the superonasal edge of the pupil and 3 CDCS scan through the inferonal pupil. This double-angle protocol ensures that acceptable Doppler angle is obtained on each retinal branch vessel in at least 1 scan. The CDCS scan data, a 3-dimensional volumetric OCT scan of the optic disc scan, and a color photograph of the optic disc are used together to obtain retinal blood flow measurement on an eye. We have developed a blood flow measurement software called Doppler optical coherence tomography of retinal circulation (DOCTORC). This semi-automated software is used to measure total retinal blood flow, vessel cross section area, and average blood velocity. The flow of each vessel is calculated from the Doppler shift in the vessel cross-sectional area and the Doppler angle between the vessel and the OCT beam. Total retinal blood flow measurement is summed from the veins around the optic disc. The results obtained at our Doppler OCT reading center showed good reproducibility between graders and methods (2>0.57 with visual field pattern deviation). Doppler OCT is a new method to perform rapid, noncontact, and repeatable measurement of total retinal blood flow using widely available Fourier-domain OCT instrumentation. This new technology may improve the practicality of making these measurements in clinical studies and routine clinical practice.

Original languageEnglish (US)
JournalJournal of Visualized Experiments
Issue number67
DOIs
StatePublished - Sep 18 2012

Fingerprint

Optical tomography
Optical Coherence Tomography
Blood
Optic Disk
Flow measurement
Optics
Doppler Effect
Retinal Vessels
Doppler effect
Pupil
Software
Clinical Protocols
Visual Fields
Reading
Veins
Color
Technology

Keywords

  • Doppler optical coherence tomography
  • Dual circular scan pattern
  • Image analysis
  • Issue 67
  • Medicine
  • Ophthalmology
  • Optic disc
  • Physics
  • Semi-automated grading software
  • Total retinal blood flow

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Doppler optical coherence tomography of retinal circulation. / Tan, Ou; Wang, Yimin; Konduru, Ranjith K.; Zhang, Xinbo; Sadda, Srinivas R.; Huang, David.

In: Journal of Visualized Experiments, No. 67, 18.09.2012.

Research output: Contribution to journalArticle

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