TY - JOUR
T1 - Dopaminergic retinal cell differentiation in culture
T2 - Modulation by forskolin and dopamine
AU - Guimarães, Marília Zaluar P.
AU - Hokoç, Jan Nora
AU - Duvoisin, Robert
AU - Reis, Ricardo A.M.
AU - De Mello, Fernando Garcia
PY - 2001
Y1 - 2001
N2 - We examined the effects of dopamine and cAMP on the differentiation of dopaminergic retinal cells in the chick retina, using an in vitro system and tyrosine hydroxylase immunocytochemistry. Tyrosine hydroxylase-positive cells were detected in cultures prepared from embryonic day 10 retinas. These increased in number as a function of time in vitro and by treatment for 4 days with forskolin. Besides causing a 3.4-fold increase in the tyrosine hydroxylase-positive population, forskolin also caused these cells to developed morphogenetic features of more mature cells. As opposed to forskolin, cultures treated with dopamine exhibited a 55% reduction of the tyrosine hydroxylase-positive cell population, as compared to untreated cultures. Quinpirole was able to mimic the dopamine effect. This dopamine effect could only be blocked by clozapine, whereas raclopride and eticlopride were ineffective. Our results suggest the existence of a narrow window during development when undifferentiated dopaminergic cells are capable of being influenced by specific signals, possibly via cAMP production. The data also indicate that dopamine may act as a regulatory factor limiting the tyrosine hydroxylase-positive population in the retina.
AB - We examined the effects of dopamine and cAMP on the differentiation of dopaminergic retinal cells in the chick retina, using an in vitro system and tyrosine hydroxylase immunocytochemistry. Tyrosine hydroxylase-positive cells were detected in cultures prepared from embryonic day 10 retinas. These increased in number as a function of time in vitro and by treatment for 4 days with forskolin. Besides causing a 3.4-fold increase in the tyrosine hydroxylase-positive population, forskolin also caused these cells to developed morphogenetic features of more mature cells. As opposed to forskolin, cultures treated with dopamine exhibited a 55% reduction of the tyrosine hydroxylase-positive cell population, as compared to untreated cultures. Quinpirole was able to mimic the dopamine effect. This dopamine effect could only be blocked by clozapine, whereas raclopride and eticlopride were ineffective. Our results suggest the existence of a narrow window during development when undifferentiated dopaminergic cells are capable of being influenced by specific signals, possibly via cAMP production. The data also indicate that dopamine may act as a regulatory factor limiting the tyrosine hydroxylase-positive population in the retina.
KW - C-AMP
KW - Chick
KW - D2-like receptor
KW - Development
KW - Tyrosine hydroxylase
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U2 - 10.1046/j.0953-816X.2001.01575.x
DO - 10.1046/j.0953-816X.2001.01575.x
M3 - Article
C2 - 11403686
AN - SCOPUS:0034969651
SN - 0953-816X
VL - 13
SP - 1931
EP - 1937
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 10
ER -