Dopaminergic retinal cell differentiation in culture: Modulation by forskolin and dopamine

Marília Zaluar P Guimarães, Jan Nora Hokoç, Robert Duvoisin, Ricardo A M Reis, Fernando Garcia De Mello

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We examined the effects of dopamine and cAMP on the differentiation of dopaminergic retinal cells in the chick retina, using an in vitro system and tyrosine hydroxylase immunocytochemistry. Tyrosine hydroxylase-positive cells were detected in cultures prepared from embryonic day 10 retinas. These increased in number as a function of time in vitro and by treatment for 4 days with forskolin. Besides causing a 3.4-fold increase in the tyrosine hydroxylase-positive population, forskolin also caused these cells to developed morphogenetic features of more mature cells. As opposed to forskolin, cultures treated with dopamine exhibited a 55% reduction of the tyrosine hydroxylase-positive cell population, as compared to untreated cultures. Quinpirole was able to mimic the dopamine effect. This dopamine effect could only be blocked by clozapine, whereas raclopride and eticlopride were ineffective. Our results suggest the existence of a narrow window during development when undifferentiated dopaminergic cells are capable of being influenced by specific signals, possibly via cAMP production. The data also indicate that dopamine may act as a regulatory factor limiting the tyrosine hydroxylase-positive population in the retina.

Original languageEnglish (US)
Pages (from-to)1931-1937
Number of pages7
JournalEuropean Journal of Neuroscience
Volume13
Issue number10
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Colforsin
Tyrosine 3-Monooxygenase
Cell Differentiation
Dopamine
Retina
Dopamine Agents
eticlopride
Population
Raclopride
Quinpirole
Clozapine
Immunohistochemistry

Keywords

  • C-AMP
  • Chick
  • D2-like receptor
  • Development
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dopaminergic retinal cell differentiation in culture : Modulation by forskolin and dopamine. / Guimarães, Marília Zaluar P; Hokoç, Jan Nora; Duvoisin, Robert; Reis, Ricardo A M; De Mello, Fernando Garcia.

In: European Journal of Neuroscience, Vol. 13, No. 10, 2001, p. 1931-1937.

Research output: Contribution to journalArticle

Guimarães, Marília Zaluar P ; Hokoç, Jan Nora ; Duvoisin, Robert ; Reis, Ricardo A M ; De Mello, Fernando Garcia. / Dopaminergic retinal cell differentiation in culture : Modulation by forskolin and dopamine. In: European Journal of Neuroscience. 2001 ; Vol. 13, No. 10. pp. 1931-1937.
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